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CAS:67-97-0
Appearance:White to yellow solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（CAL-27 and SCC-15 cells; 75 nM Vitamin D3, 37℃, 48h）：
The cell apoptosis assay adopted flow cytometry using Annexin V-FITC/PI. According to the protocol, the cells treated with ASA (1 mM) or/and vitamin D3(75 nM) were incubated for 48 h at 37℃, and then the cells were rinsed with PBS buffer twice and centrifuged for 5 min (1000 rpm). Before tested, the cells were gently resuspended in Binding Buffer and stained with Annexin V-FITC (5 ug/ml) and PI(10 ug/ml) for 15 min in the dark. The data were analyzed using FACScan system.
Reference：
Xiao TT, Li X, Feng JL, Li Y. Combined effects of aspirin and vitamin D3 on two OSCC cell lines: a preliminary study. Biotechnol Lett. 2018 Mar;40(3):551-559. doi: 10.1007/s10529-018-2508-5. Epub 2018 Jan 18. PMID: 29349624.
In Vivo:
Rat（male，6?week?old Sprague Dawley rats；subcutaneous injection/subcutaneous injection）
Following a week acclimation period, 22 rats were divided into the arterial calcification group (experimental group; n=16) and normal arterial group (control group; n=6). The animals in the arterial calcification group were injected with vitamin K 11.5 mg/(100 g body weight/day) subcutaneously from the first day to the sixth, on the third day, subcutaneous injection of vitamin D3 (Beijing Solarbio Science& Technology Co., Ltd.; 30,000 U/(100 g body weight/day) was continued once per day until the fifth day, and warfarin 150 mg/kg was administered twice per day orally until the sixth day; and normal arterial group were injected with 0.9% saline of the same volume with the same method.
Reference：
Xin H, Wang Z, Wu S, Wang P, Tao X, Xu C, You L. Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α-SMA expression and upregulating OPN expression. Exp Ther Med. 2019 Jul;18(1):705-710. doi: 10.3892/etm.2019.7626. Epub 2019 May 29. PMID: 31281450; PMCID: PMC6580105.
Rat（Sprague-Dawley (SD) rats aged 7–8 weeks；VitD3 (5 μg/kg body weight,every day)；Gavage）:
The pregnant rats were randomly divided into 4 groups, namely control group, PM2.5 group, VitD3 group, and VitD3 + PM2.5 group. The rats in the PM2.5 group received intratracheal instillation of 1.0 mg/mL COF-PM2.5 (3 mL/kg body weight, for 3 continuous days) and intragastric administration of VitD3-free vehicle (every day). Rats in the VitD3 group received intragastric administration of VitD3 (5 μg/kg body weight,every day) and intratracheal instillation of normal saline (for 3 continuous days). Rats in the VitD3 + PM2.5 group received intratracheal instillation of 1.0 mg/mL COF-PM2.5 (3 mL/kg body weight, for 3 continuous days) and intragastric administration of VitD3 (5 μg/kg body weight, every day). Rats in the control group received intratracheal instillation of normal saline (for 3 continuous days) and intragastric administration of VitD3-free vehicle (every day). The rats were anesthetized by pentobarbital and then sacrificed by cervical dislocation on GD21.
Reference：
Ding R, Li J, Zhang Q, Zhang C, Li N, Sun S, Li C, Shen C, Zhao Q, Chen H, Hao J, Cao J. Vitamin D3 protects intrauterine growth restriction induced by cooking oil fume derived fine particulate matters. Ecotoxicol Environ Saf. 2022 Jan 1;229:113103. doi: 10.1016/j.ecoenv.2021.113103. Epub 2021 Dec 17. PMID: 34929501.
这个产品怎么溶解？2022-06-28 10:33:30
Administrator可以用DMSO溶解，溶解度≥5mg/mL。暂未检测过饱和溶解度。