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CAS:57-88-5
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:HPLC≥98%
Appearance:White to off-white Solid
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Examples of using this product（for reference only）
In Vivo:
Rabbit（Male New Zealand White rabbits (2.5–3.0 kg) ，2% cholesterol-containing diet）:
Male New Zealand White rabbits (2.5–3.0 kg) were fed a 2% cholesterol-containing diet (Solarbio Bioscience & Technology Co. Ltd., Shanghai, China) for one week before surgery. Then, the rabbits were intravenously anaesthetized with pentobarbital (30 mg/kg) and ketamine (10 mg/kg). A 6F sheath was inserted into the lef common carotid artery afer intravenous administration of heparin (100 IU/kg). A 2.75×14 mm balloon dilation catheter was guided through the descending aorta into the right superfcial femoral artery with guidewire assistance, infated to 6–8 atmospheres and withdrawn to the femoral bifurcation with a rotating motion three times to induce efective endothelial denudation. Afer the injury, the rabbits continued to receive the high cholesterol diet until being killed. Plasma cholesterol was then measured by an automated analyser.
Reference：
Yao J, Zhao X, Tan F, Cao X, Guo S, Li X, Huang Z, Diabakte K, Wang L, Liu M, Shen Z, Li B, Cao Z, Sheng S, Lu M, Cao Y, Jin H, Zhang Z, Tian Y. Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits. Sci Rep. 2020 Jul 15;10(1):11638. doi: 10.1038/s41598-020-68543-9. PMID: 32669704; PMCID: PMC7363872.
Mice（Healthy male C57BL/6 J mice (8 weeks old, 18–20 g) ，1.25% cholesterol）:
Healthy male C57BL/6 J mice (8 weeks old, 18–20 g) After adaptive feeding for 2 weeks, 60 mice were divided randomly into 6 groups, with 10 mice in each group: LD 0-week group, LD 4-week group, LD 8-week group, LD+ normal saline (NS) 8-week group, regular rodent diet (RD) + NS 8-week group and LD +UDCA 8-week group. The first 3 groups received an LD for 0, 4, and 8 weeks, respectively, and the last 3 groups were given the same volume of NS or UDCA (60 mg/kg/d, once a day) at 9:00 am for 8 consecutive weeks. The “0 + LD” group and “RD + NS” group represent the control groups. The “8 + LD” group and “LD + NS” group represent the model groups.
The LD consisted of 80.25% regular rodent diet, 0.50% cholic acid, 1.25% cholesterol (Beijing Solarbio Science & Technology, China), 16% butterfat and 2% corn oil.
Reference：
Fan N, Meng K, Zhang Y, Hu Y, Li D, Gao Q, Wang J, Li Y, Wu S, Cui Y. The effect of ursodeoxycholic acid on the relative expression of the lipid metabolism genes in mouse cholesterol gallstone models. Lipids Health Dis. 2020 Jul 2;19(1):158. doi: 10.1186/s12944-020-01334-3. PMID: 32615989; PMCID: PMC7333299.
In Vitro:
Liposome preparation:
Precisely weighed 120 mg of L-α-phosphatidylcholine(Solarbio, Beijing, China), 40 mg of cholesterol (Solarbio),20 mg of DSPE-PEG-FA, and 3 mg of erastin were fully dissolved in 5 mL chloroform and mixed with 3 mL of 1 mg/mL erastin chloroform solution. Chloroform was then removed using a rotary evaporator to form a dry-lipid film at 35 ℃ under uniform speed. MT1DP (300 nM) dissolved in phosphate-buffered saline (PBS) (pH 7.4, 5 mL) was added to the lipid film and hydrated for 60 min. After the hydration was completed, the cells were sonicated with an ultrasonic cell disruptor and passed through a polyethersulfone membrane with a membrane pore diameter of 450 and 220 nm to decrease the particle size.
Reference：
Gai C, Liu C, Wu X, Yu M, Zheng J, Zhang W, Lv S, Li W. MT1DP loaded by folate-modified liposomes sensitizes erastin-induced ferroptosis via regulating miR-365a-3p/NRF2 axis in non-small cell lung cancer cells. Cell Death Dis. 2020 Sep 14;11(9):751. doi: 10.1038/s41419-020-02939-3. PMID: 32929075; PMCID: PMC7490417.
