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CAS:147-94-4
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（K562 cells；0.1 ng/mL cytarabine；5d）:
K562 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 1% penicillin and streptomycin. K562 cells were differentiated by erythroid induction at an initial concentration of 1×105/mL cells. A final concentration of 40 μM hemin (Solarbio, China) and 0.1 ng/mL cytarabine (Solarbio, China) were added to the medium to induce erythroid differentiation, and the cells were placed at 37℃ under hypoxic (5% CO2, 5% O2), CoCl (200 μM) and normoxic (5% CO2, 20% O2) conditions for 5 days.
Reference：
Gao Z, Li Z, Li X, Xiao J, Li C. Regulation of erythroid differentiation in K562 cells by the EPAS1-IRS2 axis under hypoxic conditions. Front Cell Dev Biol. 2023 Jun 1;11:1161541. doi: 10.3389/fcell.2023.1161541. PMID: 37325570; PMCID: PMC10267359.
In Vivo:
Mice（50~200 mg/kgAra-C，I.P，7d）:
After a week to adapt to the facility, mice were randomly divided into four groups (n = 10 mice per group) for intestinal pathology and survival analysis in the selection of the optimal doses of Ara-C. Ara-C and AS-IV were dissolved by 0.9% saline and dimethyl sulfoxide(DMSO) respectively. The control group was injected intraperitoneally with 0.9% saline (5 ml/kg) and the Ara-C group was intraperitoneally injected with different doses of Ara-C (50 mg/kg, 100 mg/kg, 200 mg/kg) for 7 days. Mice were killed at day 10.
Reference：
Li JJ, Li YL, Chu W, Li GQ, Zhang M, Dong JJ, Li L, Li CH, Zhang JB, Li JW, Jin XJ, Liu YQ. Astragaloside IV alleviates cytarabine-induced intestinal mucositis by remodeling macrophage polarization through AKT signaling. Phytomedicine. 2023 Jan;109:154605. doi: 10.1016/j.phymed.2022.154605. Epub 2022 Dec 15. PMID: 36610133.
