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CAS:50-02-2
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Dexamethasone is a basic agent for osteogenesis induction, which can activate glucocorticoid receptors located on the surface of stem cells and significantly increase the activity of alkaline phosphatase. It can induce stem cells to differentiate into osteoblasts.
This product can be used for induction of osteoblasts and other cells.
Examples of using this product（for reference only）
In Vitro:
Cell（1 μM dexamethasone/adipogenic； 10 nM dexamethasone/Osteogenic）：
The adipogenic medium was the complete culture medium supplemented with 1 μM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio), and 0.5 mM isobutyl-methylxanthine (Solarbio).The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio).
Reference：
Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.
Cell（Osteogenic；100 nM dexamethasone）：
Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining.
Reference：
Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421.
Cell (Osteogenic：10 nM dexamethasone; adipogenic： 1 mM dexamethasone )：
Osteogenic differentiation assay：
Cells were cultured in the osteogenic medium which contained 90% a-MEM (BI), 10% FBS (BI), 10 nM dexamethasone (Solarbio,Beijing, China), 10 mM b-glycerophosphate (Solarbio) and 50 mg/L ascorbic acid (Solarbio).
Adipogenic differentiation assay：
Cells were cultured in the adipogenic medium which contained 90% a-MEM (BI), 10% FBS (BI), 1 mM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio) and 0.5 mM isobutyl-methylxanthine (Solarbio).
Reference：
Jia L, Zhang Y, Ji Y, Xiong Y, Zhang W, Wen Y, Xu X. YAP balances the osteogenic and adipogenic differentiation of hPDLSCs In Vitro partly through the Wnt/β-catenin signaling pathway. Biochem Biophys Res Commun. 2019 Oct 8;518(1):154-160. doi: 10.1016/j.bbrc.2019.08.024. Epub 2019 Aug 14. PMID: 31421821.
Cell（BMSCs and HUVECs；10 μM or 100 μM dexamethasone/DEX）：
The cells were used between the third and sixth passages (p3–p6) and divided into six groups for the cell counting kit-8 (CCK-8) assay: (1) control; (2) dexamethasone (DEX; 10 μM; Solarbio Co., Ltd.; concentration was selected based on previous studies); (3) DEX + 5 μM AS-IV; (4) DEX + 20 μM AS-IV; (5) DEX + 50 μM AS-IV; and (6) DEX + 100 μM AS-IV. For alizarin red staining; alkaline phosphatase (ALP) staining; and apoptosis, tube formation, scratch wound and Transwell migration assays, the cells were divided into control, DEX, DEX + AS-IV and DEX + AS-IV + LY294002 groups. DEX concentration was 10 μM for alizarin red staining; ALP staining; and tube formation, Transwell migration and scratch wound assays; and 100 μM for the apoptosis assay. AS-IV and DEX were administered simultaneously. The concentrations of AS-IV and LY294002 were 50 and 10 μM, respectively.
Reference：
Shan H, Lin Y, Yin F, Pan C, Hou J, Wu T, Xia W, Zuo R, Cao B, Jiang C, Zhou Z, Yu X. Effects of astragaloside IV on glucocorticoid-induced avascular necrosis of the femoral head via regulating Akt-related pathways. Cell Prolif. 2023 Apr 26:e13485. doi: 10.1111/cpr.13485. Epub ahead of print. PMID: 37186483.
能溶于水吗2023-09-25 15:11:01
AdministratorDexamethasone地塞米松不溶于水。