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CAS:476-66-4
Storage:Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:HPLC≥98%
Appearance:Yellow to brown Solid
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Examples of using this product（for reference only）
In Vitro
Cell viability
Firstly, passages 3 chondrocytes were seeded into 96-well plates (5,000 cells/well) and incubated for 24 h. Then, the cells incubated with a concentration gradient (12.5, 25, 50 μM) of Ellagic acid (EA) for 24 h and 48 h when the cells reached a confluency of 90% -- 95%. Next, half the cells were incubated in IL-1β (10 ng/ml) for the next 24 h.Finally, 10 μl CCK-8 solution was added to each well for 2 h and the optical density was observed at 450 nm with a spectrophotometer . All experiments were repeated three times.
Source :Lin Z, Lin C, Fu C, Lu H, Jin H, Chen Q, Pan J. The protective effect of Ellagic acid (EA) in osteoarthritis: An In Vitro
and In Vivo
study. Biomed Pharmacother. 2020 May; 125:109845. Doi: 10.1016 / j.b iopha. 2020.109845. Epub 2020 Feb 12. PMID: 32058211.
In Vivo
Mice
The mouse osteoarthritis model was established by surgical destabilization of the medial meniscus (DMM). In brief, mice were anesthetized with intraperitoneal injection of 2 % (w/v) pentobarbital (40 mg kg?1,) and then the joint capsule of right knee medial to the patellar tendon was excised. The medial meniscotibial ligaments were cut with microsurgical scissors. Surgery consisting of an arthrotomy was also performed on the left knee joint of mice and the joint was used as the sham group. Ultimately, all of the mice were separated into three groups randomly (n = 15 in each): a control group (sham-operated), OA group (OA) and OA treated with Ellagic acid (EA) group.
Source :Lin Z, Lin C, Fu C, Lu H, Jin H, Chen Q, Pan J. The protective effect of Ellagic acid (EA) in osteoarthritis: An In Vitro
and In Vivo
study. Biomed Pharmacother. 2020 May; 125:109845. Doi: 10.1016 / j.b iopha. 2020.109845. Epub 2020 Feb 12. PMID: 32058211.
