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CAS:50-28-2
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（epidermal cells and skin-derived precursors (SKPs)，20 nM b-estradiol）：
Formation of HFS and IHFSAfter collected in isolation procedure, the epidermal cells and SKPs were mixed at ratio of 1:5e9. Then the mixture was seeded into 20 ml HF induction culture medium (DMEM/F12;10% (v/v) FBS, 1% (v/v) Insulin-transferrin-selenium,0.2 nM 3,30,5-Triiodo-L-thyronine, 20 ng/ml basic fibroblast growth factor, 2 nM hydrocortisone hemisuccinate, 2 nM testosterone, Solarbio, China; 20 nM b-estradiol, Solarbio, China; 20 nM progesterone, Solarbio, China) droplet on the lid of 100 mm dish with a total cell of 5×105 in each droplet. When the lid was put upside down, the cells sedimented at the bottom of the droplet and aggregated to form HFS after 24 h culture. The culture medium was changed every other day. After 7 days culture, the HFS was collected.
Reference：
Zhang Y, Enhejirigala, Li Z, Song W, Yao B, Li J, Wang Y, Duan X, Yuan X, Huang S, Fu X. The role of CTHRC1 in hair follicle regenerative capacity restored by plantar dermis homogenate. Biochem Biophys Res Commun. 2021 Sep 24;571:14-19. doi: 10.1016/j.bbrc.2021.07.056. Epub 2021 Jul 20. PMID: 34298337.
Cell（hAMSCs；1×10-6 mol/L β-estradiol；3d）：
For endometrial diferentiation, when hAMSCs reached 30–40% confuence, the medium was exchanged for DMEM/F12 supplemented with 2%FBS, 10 ng/mL TGF-β1, 10 ng/mL EGF, 10 ng/mL PDGF-BB, 1× 10-6 mol/L β-estradiol (Solarbio), and endometrial conditioned medium. Uninduced cells were cultured with DMEM/F12 containing 2% FBS. After 3 days, the expression of CK7 and CK19 was detected by immunofuorescence.
Reference：
Huang J, Zhang W, Yu J, Gou Y, Liu N, Wang T, Sun C, Wu B, Li C, Chen X, Mao Y, Zhang Y, Wang J. Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration. Stem Cell Res Ther. 2022 Jan 12;13(1):17. doi: 10.1186/s13287-021-02682-2. PMID: 35022063
