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CAS:314-13-6
Storage:Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥85%
Appearance:Reddish brown Solid
Evans Blue, also known as azo Blue, and Trypan Blue are both cellular active dyes that are commonly used to test cell membrane integrity and cell viability. Living cells cannot be stained blue by Evans blue because of their efflux, while dead cells are stained pale blue. It is therefore possible to distinguish between dead and living cells under the microscope in this way, but not between death and necrosis. Evans Blue is a non-permeable dye that, in the presence of damage to the plasma membrane, can enter the cytoplasm and nuclei and stain them blue. It can be used to check cell viability and study the permeability of the blood-brain barrier. Evans Blue is also a potent L-glutamate uptake inhibitor via the membrane-bound excitatory amino acid transporter (EAAT).
Examples of using this product（for reference only）
In Vivo:
Evans blue Post BMS or PLS implantation, ≥ three healthy SD rats were randomly selected and respectively anesthetized for periods of 1 week and 1, 3 months.
The first involved a 10% chloral hydrate by intraperitoneal injection, and then a 1 mL 2% Evans blue (Solarbio, CAS: 314-13-6) was injected by tail intravenous injection. These rats were subsequently sacrificed 1 h after the dyeing cycle at which point the heart was perfused with 0.9% saline containing one heparin sodium salt (Solarbio, CAS: 9041-08-1). At this time, blood vessels with implanted scaffolds were removed. After removing excess tissue fat, blood vessels were dissected longitudinally, fixed with 4% paraformaldehyde. Subsequently, these blood vessels were stored at 4 ℃ to be used for collecting images of the endocardium coloration.
Reference：
Yin T, et al. Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold. Bioact Mater. 2021 Aug 24;10:378-396. doi: 10.1016/j.bioactmat.2021.08.020.
In Vitro:
Cell viability（0.25% (w/v) Evans blue, 1h, 600nm）:
Root cell viability was also quantitatively evaluated by detecting the relative uptake of Evans blue. Root segments of the treated lettuce seedlings were soaked in 0.25% (w/v) Evans blue (Solarbio, Beijing, China) for 1 h at 25℃. The stained roots were washed with distilled H2O, and then soaked in 1 mL N,N-dimethylformamide for 24 h at 25℃ in darkness to extract the Evans blue that had been absorbed into the lettuce roots. Absorbance of the released Evans blue was measured at 600 nm by a spectrophotometer. The relative Evans blue uptake was calculated as the ratio between the OD values of the treated group and the control.
Reference：
Yan ZQ, Tan J, Guo K, Yao LG. Phytotoxic mechanism of allelochemical liquiritin on root growth of lettuce seedlings. Plant Signal Behav. 2020 Oct 2;15(10):1795581. doi: 10.1080/15592324.2020.1795581. Epub 2020 Jul 21.
