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CAS:3326-32-7
Appearance:Light yellow to brown Solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥97%
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Examples of using this product（for reference only）
In Vitro:
Phagocytosis Assay of Hemocyte（FITC-labeled C. albicans (1×105 cells, 5 μl)，37℃ for 3 h in dark ）:
In brief, 15 larvae per group were inoculated with 5 μl of PBS or ZnO-NP suspensions (2 mg/kg) via the last left proleg. After incubation at 37℃ for 2 h, each larva was challenged with fluorescein isothiocyanate isomer I (FITC; Solarbio, Beijing, China)-labeled C. albicans (1×105 cells, 5 μl) and incubated at 37℃ for 3 h in dark. For preparing FITC-labeled C. albicans, C. albicans suspensions (1×109 cells/ml) were incubated with 0.1 mg/ml in a shaking incubator in the dark for 1 h and washed with PBS three times. Subsequently, 50 μl of hemolymph was obtained from each larva and diluted in a 12-well plate containing 950 μl of IPS and 0.002% PTU. The plate was gently shaken followed by standing for 15 min at room temperature. The phagocytosis of C. albicans by hemocytes was visualized using an IX73 fluorescence microscope.
Reference：
Xu MN, Li L, Pan W, Zheng HX, Wang ML, Peng XM, Dai SQ, Tang YM, Zeng K, Huang XW. Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans. Front Microbiol. 2021 Dec 10;12:766138. doi: 10.3389/fmicb.2021.766138. PMID: 34956129; PMCID: PMC8702860.
