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CAS:108321-42-2
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥98%
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It is an aminoglycoside antibiotic and is the most commonly used resistance screening reagent for stable transfection. By inhibiting the gene of transposon Tn601 and Tn5, it interferes with ribosome function and blocks protein synthesis, and produces toxins to prokaryotic and eukaryotic cells. When neo gene is integrated into the DNA of eukaryotic cells, the sequence encoded by neo gene can be transcribed into mRNA, thus obtaining the efficient expression of the resistance product aminoglycoside phosphotransferase, so that the cells can acquire resistance and grow in selective medium containing G418.
Examples of using this product（for reference only）
In Vitro:
Cell（HepG2.2.15 cells，380 μg/mL G-418 sulfate）:
Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Solarbio) and supplemented with 10% fetal bovine serum (FBS).G-418 sulfate (380 μg/mL; Solarbio) was added to DMEM with 10% FBS to maintain HepG2.2.15 cells. All cells were incubated at 37℃ with 5% CO2.
Reference：
Guo C, Ouyang Y, Cai J, Xiong L, Chen Y, Zeng X, Liu A. High expression of IL-4R enhances proliferation and invasion of hepatocellular carcinoma cells. Int J Biol Markers. 2017 Oct 31;32(4):e384-e390. doi: 10.5301/ijbm.5000280. PMID: 28665449.
Cell（（HepG2.2.15 cells，300 μg/mL G-418 ）:
HepG2.2.15 cells were incubated in minimum Eagle’s medium containing 10% FBS and 300 μg/mL G418 (Solarbio, Beijing, China).
Reference：
Jiang W, Wang L, Zhang Y, Li H. Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/TNFAIP3 Axis. Cancer Manag Res. 2020 Nov 2;12:11031-11040. doi: 10.2147/CMAR.S272983. PMID: 33173336; PMCID: PMC7648158.
Cell（OC cells，800 μg/mL G418）:
PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Solarbio, Shanghai, China) at a concentration of 800 μg/mL and puromycin (Solarbio, Shanghai, China) at a concentration of 2 μg/mL.
Reference：
Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.
Cell（Replicon cell ，500 μg/ml G418）:
Replicon cell lines were selected and maintained in 500 μg/ml G418 (Geneticin; Solarbio, China).
Reference：
Sobhanimonfared F, Bamdad T, Roohvand F. Cross talk between alcohol-induced oxidative stress and HCV replication. Arch Microbiol. 2020 Sep;202(7):1889-1898. doi: 10.1007/s00203-020-01909-9. Epub 2020 May 24. PMID: 32448963.
Cell（A549, H446 and 95-D cells, 500 μg/mL G418）:
To generate P65 overexpression cell lines, the full length of p65 cDNA was inserted into pCMV-Flag vector and transfected into A549, H446 and 95-D cells, then selected with 500 μg/mL G418 (Solarbio, IG0010) to generate p65 stable expression cell lines.
Reference：
Zeng C, Zou T, Qu J, Chen X, Zhang S, Lin Z. Cyclovirobuxine D Induced-Mitophagy through the p65/BNIP3/LC3 Axis Potentiates Its Apoptosis-Inducing Effects in Lung Cancer Cells. Int J Mol Sci. 2021 May 29;22(11):5820. doi: 10.3390/ijms22115820. PMID: 34072333; PMCID: PMC8199090.
请问可以高温灭菌吗2023-10-11 17:16:28
Administrator建议使用0.22μm以下的有机滤膜进行过滤除菌。