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CAS:517-28-2
Appearance:Light yellow to brown Solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Hematoxylin is an alkaline dye that is oxidized to form hematoxylin, which is used with mordant to stain the cell nucleus.
Three common hematoxylin preparation methods (for reference only)
Harris’ hematoxylin
To prepare, 2.5g hematoxylin is dissolved in 25mL anhydrous alcohol. Take a 2000mL triangular flask, pour 50g potassium alum into it, add 500mL distilled water, heat it on an electric furnace, dissolve the potassium alum, after it is completely dissolved, when the temperature reaches about 90℃, add the pre-dissolved alcohol hematoxylin solution, and continue to heat it until it boils for 3 to 5min. At this time, the color of the solution gradually deepens and becomes purple red. Remove the power supply. Add 1.25g yellow mercury oxide, when fully oxidized, plug back in the power and continue heating for 3 to 5min, at this time the color of the solution turns dark purple, unplug the power, directly insert the triangle flask into the prepared ice water, put it in the dark, filter it the next day and add 20mL acetic acid, you can use for three months.
Note:
1, hematoxylin must be dissolved in advance, otherwise it is difficult to completely dissolve;
2, adding yellow mercury oxide, can produce a large number of bubbles, therefore, the preparation of 500mL solution, must choose more than 2000mL container, otherwise the liquid will overflow
3, when the solution becomes deep purple, the oxidation should be terminated, and the flask should be directly inserted into the ice water. The main purpose of rapid cooling is not to over-oxidize the solution, so as not to shorten the service life of the solution
4. Add glacial acetic acid after solution filtration. Don’t turn it upside down
5, the hematoxylin in use for a period of time, can produce a metal film on the liquid surface. When this phenomenon occurs, it can be removed with coarse filter paper, otherwise it will be difficult to remove it on the slice;
6, hematoxylin after adding glacial acetic acid, its PH value is between 2 and 2.28.
Mayer hematoxylin
This reagent is often used for the staining of conventional sections, and it will not overstain the nucleus. The time can be 20min to 30min, and the nuclear chromatin is clearly visible, and the nucleus is gray and black. It can be used as a contrast dyeing reagent for special colored nuclei. When preparing the solution, 100mL distilled water was heated slightly, 0.1g hematoxylin was added and stirred continuously until completely dissolved, then 5g potassium aluminum sulfate was added to make it fully dissolved, 0.1 citric acid and 5g chloral hydrate were added, and then 20mg sodium iodate was added after all dissolved, stirring evenly, and the color of the solution turned to dark brown. Strain and use.
Gill modified hematoxylin
To prepare, 2g hematoxylin was first dissolved in 250mL anhydrous alcohol, then 17.6g aluminum sulfate was dissolved in 750mL distilled water, then the two liquids were mixed and 0.2-0.3g sodium iodate was added, and finally 15-25ml glacial acetic acid was added. This solution is semi-oxidized progressive hematoxylin solution, which does not produce precipitation and has little oxide film.
