CAS:78281-02-4
Storage:Powder:2-8℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year
Purity:HPLC≥98%
Appearance:Light yellow to yellow Solid
Examples of using this product(for reference only)
In Vitro:
Cell(Chondrocytes;2.5~ 40 μM HSYA;24h):
For the In Vitro study, to assess the anti-inflammatory effects of different concentrations of HSYA, cells were treated with 10 ng mL?1 IL-1β alone or in combination with HSYA pretreatment at different concentrations (2.5, 10, or 40 μM). A control group was left untreated except for medium changes. Cells were harvested after 24 h of incubation. To investigate the activation of NF-κB and MAPKs, the duration of IL-1β stimulation was 2 h. Meanwhile, to explore functional changes, such as inflammatory or ECM markers, the duration was extended to 24 h.
Reference:
Hu ZC, Xie ZJ, Tang Q, Li XB, Fu X, Feng ZH, Xuan JW, Ni WF, Wu AM . Hydroxysafflor yellow A (HSYA) targets the NF-κB and MAPK pathways and ameliorates the development of osteoarthritis. Food Funct. 2018 Aug 15;9(8):4443-4456. doi: 10.1039/c8fo00732b. PMID: 30070297.
In Vivo:
Mice(ten-week-old C57BL/6 male wild-type (WT) mice; 20 mg/kg/day HSYA; I.P,1/d,8 week):
After DMM, the HSYA treatment groups received HSYA administration for 20 mg/kg/day (the HSYA was dissolved in saline and injected intraperitoneally once a day for eight consecutive weeks). Meanwhile, the mice in the DMM alone group received an equivalent volume of saline. All animals were sacrificed after eight weeks post-surgery, and cartilage tissue samples were collected for iconographic and histological analysis.
Reference:
Hu ZC, Xie ZJ, Tang Q, Li XB, Fu X, Feng ZH, Xuan JW, Ni WF, Wu AM . Hydroxysafflor yellow A (HSYA) targets the NF-κB and MAPK pathways and ameliorates the development of osteoarthritis. Food Funct. 2018 Aug 15;9(8):4443-4456. doi: 10.1039/c8fo00732b. PMID: 30070297.