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CAS:78281-02-4
Appearance:Light yellow to yellow solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro:
Cell（Chondrocytes；2.5~ 40 μM HSYA；24h）：
For the In Vitro study, to assess the anti-inflammatory effects of different concentrations of HSYA, cells were treated with 10 ng mL?1 IL-1β alone or in combination with HSYA pretreatment at different concentrations (2.5, 10, or 40 μM). A control group was left untreated except for medium changes. Cells were harvested after 24 h of incubation. To investigate the activation of NF-κB and MAPKs, the duration of IL-1β stimulation was 2 h. Meanwhile, to explore functional changes, such as inflammatory or ECM markers, the duration was extended to 24 h.
Reference：
Hu ZC, Xie ZJ, Tang Q, Li XB, Fu X, Feng ZH, Xuan JW, Ni WF, Wu AM . Hydroxysafflor yellow A (HSYA) targets the NF-κB and MAPK pathways and ameliorates the development of osteoarthritis. Food Funct. 2018 Aug 15;9(8):4443-4456. doi: 10.1039/c8fo00732b. PMID: 30070297.
In Vivo:
Mice（ten-week-old C57BL/6 male wild-type (WT) mice; 20 mg/kg/day HSYA; I.P，1/d，8 week）：
After DMM, the HSYA treatment groups received HSYA administration for 20 mg/kg/day (the HSYA was dissolved in saline and injected intraperitoneally once a day for eight consecutive weeks). Meanwhile, the mice in the DMM alone group received an equivalent volume of saline. All animals were sacrificed after eight weeks post-surgery, and cartilage tissue samples were collected for iconographic and histological analysis.
Reference：
Hu ZC, Xie ZJ, Tang Q, Li XB, Fu X, Feng ZH, Xuan JW, Ni WF, Wu AM . Hydroxysafflor yellow A (HSYA) targets the NF-κB and MAPK pathways and ameliorates the development of osteoarthritis. Food Funct. 2018 Aug 15;9(8):4443-4456. doi: 10.1039/c8fo00732b. PMID: 30070297.
