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CAS:103476-89-7
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥98%
Appearance:White to off-white Solid
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Examples of using this product（for reference only）
In Vitro:
Western blotting. （1 μM Leupeptin）:
MCF-7 cells were treated with the aforementioned CoCl2 concentrations for 24 h and cells were lysed with ice-cold radioimmunoprecipitation assay buffer (150 mM NaCl, 1.0% NP-40, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS and 50 mM Tris-HCl, pH 8.0) and protease inhibitors (AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China).
Reference：
Li Q, Ma R, Zhang M. CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells. Oncol Lett. 2018 Jan;15(1):1119-1124. doi: 10.3892/ol.2017.7369. Epub 2017 Nov 8. PMID: 29391899; PMCID: PMC5769392.
Western Blotting（0.5 ug/mL Leupeptin）:
The mice heart (n=3) was dissected out and homogenized with a homogenizer at 4 ℃ in ice-cold lysis buffer containing 150 mM NaCl, 50mM Tris (pH 7.4), 1% sodium deoxycholate, 1%TritonX-100, 0.1% SDS, 25 mM β-glycerophosphate, 2m M sodium pyrophosphate, 1mM EDTA, 1mM Na3VO4 and 0.5 ug/ml leupeptin (Solarbio, Beijing, China). The homogenate was centrifuged at 12,000×g for ten minutes at 4 ℃ to obtain the supernatant which was used to analyze the protein expression.
Reference：
Jing L, Shao J, Sun W, Lan T, Jia Z, Ma H, Wang H. Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia. Life Sci. 2020 May 1;248:116481. doi: 10.1016/j.lfs.2019.05.037. Epub 2019 May 15. PMID: 31102744.
