CAS:103404-75-7
Storage:Powder:2-8℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year
Purity:HPLC≥98%
Appearance:Light yellow to yellow Solid
Examples of using this product(for reference only)
In Vivo:
Mice(150 mg/kg D-fluorescein;intravenous/intraperitoneal injection):
To track transplanted cells In Vivo, ERCs of passage 2 were transfected with a lentiviral vector that carried the ubiquitin promoter encoding luciferase with an optimum multiplicity of infection (MOI = 50). Then, ERCs were screened twice with 2mg/mL puromycin after transfection. As previously described, theFluc activity of different ERCs numbers and the fate of transplanted ERCs were observed in living colitis mice using the IVIS Lumina Imaging System. After intravenous/intraperitoneal injection of the reporter probe D- fluorescein (150 mg/kg, IL0230, Solarbio), the animals were photographed with the IVIS Lumina imaging system for 1- 10 min. Bioluminescence signal intensity images were analyzed and processed using live image software.
Reference:
Shao B, Ren SH, Wang ZB, Wang HD, Zhang JY, Qin H, Zhu YL, Sun CL, Xu YN, Li X, Wang H. CD73 mediated host purinergic metabolism in intestine contributes to the therapeutic efficacy of a novel mesenchymal-like endometrial regenerative cells against experimental colitis. Front Immunol. 2023 Apr 25;14:1155090. doi: 10.3389/fimmu.2023.1155090. PMID: 37180168; PMCID: PMC10167049.