Products
D-Luciferin Sodium salt

CAS:103404-75-7

Storage:Powder:2-8℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year

Purity:HPLC≥98%

Appearance:Light yellow to yellow Solid

D-Luciferin Sodium salt
Cat.No:
IL0230
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Examples of using this product(for reference only)

In Vivo:

Mice(150 mg/kg D-fluorescein;intravenous/intraperitoneal injection):

To track transplanted cells In Vivo, ERCs of passage 2 were transfected with a lentiviral vector that carried the ubiquitin promoter encoding luciferase with an optimum multiplicity of infection (MOI = 50). Then, ERCs were screened twice with 2mg/mL puromycin after transfection. As previously described, theFluc activity of different ERCs numbers and the fate of transplanted ERCs were observed in living colitis mice using the IVIS Lumina Imaging System. After intravenous/intraperitoneal injection of the reporter probe D- fluorescein (150 mg/kg, IL0230, Solarbio), the animals were photographed with the IVIS Lumina imaging system for 1- 10 min. Bioluminescence signal intensity images were analyzed and processed using live image software.

Reference:

Shao B, Ren SH, Wang ZB, Wang HD, Zhang JY, Qin H, Zhu YL, Sun CL, Xu YN, Li X, Wang H. CD73 mediated host purinergic metabolism in intestine contributes to the therapeutic efficacy of a novel mesenchymal-like endometrial regenerative cells against experimental colitis. Front Immunol. 2023 Apr 25;14:1155090. doi: 10.3389/fimmu.2023.1155090. PMID: 37180168; PMCID: PMC10167049.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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