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CAS:1115-70-4
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro：
Cell （HepG2 cells，3.5 mg/L Metformin，24h）:
HepG2 cells were seeded at a density of 5000 cells/well in 100 μL of DMEM medium in 96-well plates. HepG2 cell culture was maintained in a humidified atmosphere of 5% CO2 at 37℃. After incubated for 12 h, the cells were washed with PBS and the medium was replaced with DMEM high glucose medium supplemented with FBS (2%) and insulin (0.5×10-7 mol/L) and incubated for 36 h to build insulin-resistant HepG2 cells model. Subsequently, the cells were washed with serum-free DMEM high glucose medium and treated with different concentrations (0.1, 0.2, 0.4, and 0.8 mg/mL) of Up-3, Up-4, and Up-5. Medium and metformin (3.5 mg/L) were used as the normal and metformin group, respectively. After incubated for 24 h, the free supernatants of the cells were collected, and then the glucose concentration of supernatants was measured by a glucose test kit following the manufacturer’s protocol.
Reference：
Zhong QW, Zhou TS, Qiu WH, Wang YK, Xu QL, Ke SZ, Wang SJ, Jin WH, Chen JW, Zhang HW, Wei B, Wang H. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida. Food Chem. 2021 Mar 30;341(Pt 1):128148. doi: 10.1016/j.foodchem.2020.128148. Epub 2020 Sep 22. PMID: 33038776.
In Vivo：
Mice（Eight-week-old Kunming mice (approximately 20–22 g, specific pathogen-free, SPF) ，200mg/kg，4h，Oral）:
Mice were orally treated with MET(200 mg/kg) for 4 h. Blood samples were subsequently taken from the angular vein.
Reference：
Zhang W, Gao J, Shen F, Ma X, Wang Z, Hou X, Hao E, Hou Y, Bai G. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci. 2020 Oct 1;258:118151. doi: 10.1016/j.lfs.2020.118151. Epub 2020 Jul 26. PMID: 32726661.
