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My CartCAS：298-93-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：Light yellow to yellow Solid
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| CAS | 298-93-1 |
| Name | MTT |
| Molecular Formula | C18H16BrN5S |
| Molecular Weight | 414.32 |
| Solubility | Soluble in Water/DMSO |
| Purity | ≥98% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 206-069-5 |
| MDL | MFCD00011964 |
| SMILES | CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-] |
| InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| InChI | InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1 |
| PubChem CID | 64965 |
| Background | MTT is a colorimetric reagent widely used to measure cell proliferation. |
| Biological Activity | MTT是一种广泛用于测量细胞增殖的比色剂。 MTT在活细胞中从黄色还原为紫色。 |
| In Vitro | MTT与罗丹明B联合使用以测量线粒体膜电位。 MTT-formazan在线粒体中产生，作为罗丹明的荧光猝灭剂，根据膜电位分布在活细胞膜上。在没有mPMS的情况下，MTT的细胞减少很强。当MTT掺入大的单层脂质体中时，它是不透膜的，因此它通过内吞作用被细胞摄取[1]。 |
| Data Literature Source | [1]. Berridge MV，et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52. |
| Unit | Bottle |
| Specification | 200mg 10mM*1mL in Water 10mM*1mL in DMSO 5mg/ml*1mL in Water 500mg 1g |
MTT is a colorimetric agent widely used to measure cell proliferation. MTT can be reduced by some dehydrogenases in the mitochondria to produce the crystalline dark purple product formazan, which can be completely dissolved by DMSO, and then the absorbance near the wavelength of 490nm can be measured by enzymoleter. The more cells proliferate and the faster, the higher the absorbance; The greater the cytotoxicity, the lower the absorbance. Note: MTT solution should be kept away from light, long time light will cause failure. Do not use when the color turns grayish green.
Instructions for use (for reference only)
1. Collect logarithmic cells and culture cells according to their own experimental needs.
2. Carefully absorb the supernatant, add 90μL fresh medium, then add 10μL MTT solution, and continue to culture for 4 h.
3. Then absorb the supernatant, add 110μL DMSO to each hole, and oscillate at a low speed for 10 min on a shaking table to fully dissolve the crystal.
4. The light absorption value of each hole was measured at 490 nm by ELISA. Note: The commonly used concentration of MTT solution is 0.5% (for reference only), and the experimenters can also adjust it according to their own experimental needs.
Examples of using this product（for reference only）
In Vitro:
Cell （HEK-293 cells，37 ℃ ，4 h，490nm）:
Cell viability was determined by a quantitative colorimetric assay with MTT. To screen the pre-protection of SDAP1 and SDAP2, cells were pretreated with SDAP1 and SDAP2 (0.25, 0.5, 0.75, 1, 1.25, 1.5 mg/mL) before administration of GM (3 mg/mL) for 24 h. HEK-293 cells were dispensed in 96-well plates at a density of 8,000 cells/well and cultured at 37 ℃ for 24 h. Afer 24 h incubation, cells were treated with various concentrations of SDAP1 and SDAP2 (0.25, 0.5, 0.75, 1, 1.25 and 1 mg/mL) at 24 h, and aferwards exposed to GM (3 mg/mL) for 24 h. Next, afer 24 h of GM treatment, the MTT solution was added to each well, and incubated at 37 ℃ for 4 h. Te medium was removed, and 150 μL of dimethylsulfoxide (Solarbio, China) was added to each well. Finally, the optical density was detected with a microplate reader at 490 nm.
Reference：
Wang Z, Wang L, Wang J, Luo J, Ruan H, Zhang J. Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway. Sci Rep. 2020 Sep 24;10(1):15601. doi: 10.1038/s41598-020-71943-6. PMID: 32973191; PMCID: PMC7518274.
Cell（HepG2 Cell，5 mg/mL MTT，4h, 570nm）：
Methylthiazole tetrazolium (MTT) assay was applied to measure the cell viability of Up-3, Up-4, and Up-5. Briefly, after incubation, the cells was washed with PBS, and incubated with 10 μL of MTT (5 mg/mL) for 4 hours. The supernatants was mixed with 100 μL of DMSO and then the absorbance was measured at 570 nm with a microplate reader. The absorbance of the untreated cells (Normal group) was considered as 100%.
Reference：
Zhong QW, Zhou TS, Qiu WH, Wang YK, Xu QL, Ke SZ, Wang SJ, Jin WH, Chen JW, Zhang HW, Wei B, Wang H. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida. Food Chem. 2021 Mar 30;341(Pt 1):128148. doi: 10.1016/j.foodchem.2020.128148. Epub 2020 Sep 22. PMID: 33038776.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Zhong QW, et al. Food Chem. 2021 Mar 30;341(Pt 1):128148.
Ma L, et al. ACS Applied Materials & Interfaces, 2020.
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