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CAS:33069-62-4
Storage:Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:HPLC≥98%
Appearance:White to off-white Solid
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Paclitaxel can cause tubulin and tubulin dimer composed of microtubules to lose dynamic balance, induce and promote tubulin polymerization, microtubule assembly, and prevent depolymerization, so as to stabilize the microtubules and inhibit the mitosis of cancer cells and prevent the induction of apoptosis, thus effectively preventing the proliferation of cancer cells and playing an anticancer role.
Examples of using this product（for reference only）
In Vitro:
Cell：
Cytotoxicity determination: The IC50 of cells exposed to PTX was determined using a Cell Counting Kit (CCK)?8 assay. In brief, cells were inoculated at a concentration of 4x103/100 μl per well and cultured at 37?C for 12 h in a 96?well plate. The cells were then maintained at 37?C with 200 μl fresh medium containing different concentrations of PTX (0?50 nM) for 48 h. Following this, the cells were incubated with 20 μl CCK?8 solution at 37?C for 24 h. Then, the optical density value of each well was detected using a microplate reader at 450 nm.
ROS检测：
For ROS detection, 5x105 cells were seeded overnight in a 6?well plate and then treated with PTX (5 nM) at 37?C for 48 h. Subsequently, 5 μM carboxy 2'?7'?dichlorofluorescein diacetate was added in Hanks' Balanced Salt Solution and incubated with cells at room temperature for 0.5 h. Fresh tissues were harvested, followed by a single cell suspension preparation. Tissue was digested with trypsin to obtain single cell suspension. Cells were then inoculated in a 6?well plate as aforementioned. The levels of intracellular peroxide were measured using a flow cytometer at 485 nm excitation and 520 nm emission to analyze the ROS generation.
Reference：
Chen M, Su J, Feng C, Liu Y, Zhao L, Tian Y. Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+cells via the Notch1 signaling pathway in ovarian cancer. Mol Med Rep. 2021 Sep;24(3):635. doi: 10.3892/mmr.2021.12274. Epub 2021 Jul 19. PMID: 34278466; PMCID: PMC8280726.
In Vivo：
mice，I.P：
Xenograft and grouping：In total, 16 male nude mice (age, 6?8 weeks; weight, 25?30 g) . Mice were housed at 25±1?C with 12?h light/dark cycles, 50±5% humidity and free access to food and water. The mice were randomly divided into four groups: i) CD44+CD117++ PTX (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); ii) CD44+CD117++ PTX + CCL20 (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); iii) CD44?CD117?+ PTX group (n=4); and iv) CD44?CD117? + PTX + CCL20 group (n=4). For xenografts, CD44+CD117+(2x106) subsets or CD44?CD117?subsets cells (2x106) suspended in 0.5 ml PBS were subcutaneously injected into the left flank of each nude mouse to establish tumors. Then, PTX (10 mg/kg) and/or CCL20 (1 mg/kg) were injected intraperitoneally simultaneously on days 1, 3 and 7. Tumor size was monitored on the 3rd, 7th, 14th, 21st and 28th days (15). At the end of the experiment and under continuous anesthesia, the animals were euthanized by an overdose of pentobarbital (125 mg/kg).
Reference：
Chen M, Su J, Feng C, Liu Y, Zhao L, Tian Y. Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+cells via the Notch1 signaling pathway in ovarian cancer. Mol Med Rep. 2021 Sep;24(3):635. doi: 10.3892/mmr.2021.12274. Epub 2021 Jul 19. PMID: 34278466; PMCID: PMC8280726.
