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CAS:53123-88-9
Appearance:White to off-white solid
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Rapamycin is a macrolide immunosuppressant, which blocks signal transduction through different cytokine receptors and blocks the progression of T lymphocytes and other cells from G1 phase to S phase, thereby exerting immunosuppressive effect. It is also a potent and specific mTOR inhibitor and autophagy activator.
Examples of using this product（for reference only）：
In Vitro：
Cell：
Wound Healing Assay： Cells at the logarithmic growth stage were placed in a six-well plate with a cell density of 1×105 cells/well and routine-cultured in an incubator at 37 ℃ with 95% humidity and 5% CO2 for 24 h. A 200-μL pipetting tip was used to vertically scratch the six-well plate to avoid tilting. The damaged or dead cells were washed away with PBS and then the 2 mL culture medium containing different concentrations of rapamycin was added to the treatment groups. The same amount of medium (culture medium and DMSO, the concentration of DMSO was the same as that of the 10?4 drug concentration group) was added to the negative control group. Photographs were taken at 0 and 24 h using a microscope and the wound healing area was calculated using ImageJ. The experiment was repeated thrice.
Reference：
Liu Y, Zhang J, Long J, Qiu X, Wang T, Wang J. The Effects of Rapamycin on the Proliferation, Migration, and Apoptosis of Human Tracheal Fibroblasts (HTrF) and Human Tracheal Epithelial Cells (HTEpiC). J Clin Med. 2022 Jan 25;11(3):608. doi: 10.3390/jcm11030608.
In Vivo：
pigeon，wing vein injection
Experimental Design：
A total of 90 pairs of breeding pigeons were randomly assigned to 3 groups. Each pair of breeders fed 4 young squabs (“2+4” feeing pattern). On the first day of the lactation period, the breeding pigeons were randomly subjected to one of the following treatments for 7 days: sham treatment (RAPA vehicle at an equivalent volume, control), low-dose RAPA (Solarbio,Beijing, China; 0.6 mg/kg body weight (BW), dissolved in absolute ethanol and diluted with 0.85% physiological saline, once daily at 09:00 hours, wing vein injection) and high-dose RAPA (1.2 mg/kg BW).
Reference：
Chen MJ, Fu Z, Jiang SG, Wang XQ, Yan HC, Gao CQ. Targeted disruption of TORC1 retards young squab growth by inhibiting the synthesis of crop milk protein in breeding pigeon (Columba livia). Poult Sci. 2020 Jan;99(1):416-422. doi: 10.3382/ps/pez513. Epub 2019 Dec 30. PMID: 32416826; PMCID: PMC7587900.
mice，I.P：
Mice were transfected with AdIGFBPrP1 into liver tissue via the tail vein; the recombinant adenoviral vector encoding IGFBPrP1 was synthesized. Concurrently, LY294002 (4?mg/kg) and rapamycin (2?mg/kg, Beijing Solarbio) were intraperitoneally injected for one month. Both doses were determined by pre-experimental results. The mice were sacrificed at 1, 2, 4, 8, and 12 weeks (n?=?8), and liver tissues were harvested.
Reference：
Huang TJ, Ren JJ, Zhang QQ, Kong YY, Zhang HY, Guo XH, Fan HQ, Liu LX. IGFBPrP1 accelerates autophagy and activation of hepatic stellate cells via mutual regulation between H19 and PI3K/AKT/mTOR pathway. Biomed Pharmacother. 2019 Aug;116:109034. doi: 10.1016/j.biopha.2019.109034. Epub 2019 May 29. PMID: 31152924.
