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CAS:58-22-0
Appearance:White to off-white solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:HPLC≥98%
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Examples of using this product（for reference only）
In Vitro::
Cell（HUMSCs；1 mM testosterone）:
Male germ-like cell differentiation of HUMSCsThe HUMSCs used in each experiment were from passages 3–5. The male germ-like cell differentiation medium consisted of HDMEM-F12 supplemented with 5% FBS, 50% fi ltered testicular cell conditioned medium, 2 mM all-trans retinoic acid and 1 mM testosterone (Solarbio,Beijing, China). HUMSCs were grown in differentiation medium for 7, 14, or 21 days; half of the differentiation induction medium was replaced every 3–4 days. HUMSCs of the control group were cultured in normal medium (DMEM-F12 containing 5% FBS and 2% penicillin/streptomycin).
Reference：
Xie L , Zhou G , Ma L, et al. Differential expression profiles of lncRNAs and mRNAs during male germ-like cell differentiation in human umbilical mesenchymal stem cells by RNA-seq[J]. 2021.
Cell（epidermal cells and skin-derived precursors (SKPs)，2 nM testosterone）:
Formation of HFS and IHFSAfter collected in isolation procedure, the epidermal cells and SKPs were mixed at ratio of 1:5e9. Then the mixture was seeded into 20 ml HF induction culture medium (DMEM/F12;10% (v/v) FBS, 1% (v/v) Insulin-transferrin-selenium,0.2 nM 3,30,5-Triiodo-L-thyronine, 20 ng/ml basic fibroblast growth factor, 2 nM hydrocortisone hemisuccinate, 2 nM testosterone, Solarbio, China; 20 nM b-estradiol, Solarbio, China; 20 nM progesterone, Solarbio, China) droplet on the lid of 100 mm dish with a total cell of 5 x105 in each droplet. When the lid was put upside down, the cells sedimented at the bottom of the droplet and aggregated to form HFS after 24 h culture. The culture medium was changed every other day. After 7 days culture, the HFS was collected.
Reference：
Zhang Y, Enhejirigala, Li Z, Song W, Yao B, Li J, Wang Y, Duan X, Yuan X, Huang S, Fu X. The role of CTHRC1 in hair follicle regenerative capacity restored by plantar dermis homogenate. Biochem Biophys Res Commun. 2021 Sep 24;571:14-19. doi: 10.1016/j.bbrc.2021.07.056. Epub 2021 Jul 20. PMID: 34298337.
