Product Introduction:

Glycogen staining is one of the conventional staining methods in pathology. McManus first used the periodate Schiff technique in 1946 to display mucin, which is commonly used to display glycogen and other polysaccharides, as well as neutral mucinous substances and certain acidic substances, as well as cartilage, pituitary gland, fungi, pigments, amyloid substances, basement membrane, etc.The dyeing principle is that periodic acid (also known as periodic acid) is a strong oxidant that can oxidize 1,2-ethanediol groups in sugars and related substances, turning them into dialdehydes. Aldehydes can combine with Schiff reagent to form a fuchsin compound, producing a purple red color.

PAS technology is the only method that can detect different types of mucus substances (such as glycogen, mucin, and glycoprotein), but PAS technology cannot distinguish between mucin and glycogen. To accurately identify mucus substances (such as mucin and glycogen), glycogen digestion steps need to be added. Available in most cases α- Starch or malt amylase is used to catalyze the hydrolysis of glycosidic bonds of glycogen to form water-soluble disaccharides maltose, which are removed from tissue slices before applying PAS technology.

The characteristic of glycogen D-PAS staining solution is that it is treated with amylase before glycogen PAS staining. During glycogen digestion, two identical slices are required, one slice is treated with buffer containing amylase, and the other slice is treated with only buffer. Then both slices were stained with PAS method, and the disappearance of staining after digestion indicates the presence of glycogen. This kit is suitable for glycogen identification and staining of tissue paraffin and frozen sections.

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