English
中文
Product Introduction:
Early apoptosis disrupts the asymmetry of phospholipid distribution in the cell membrane, allowing phosphatidylserine (PS), which is normally located inside the cell membrane, to be exposed from inside the cell membrane to outside the cell membrane. Annexin V has a high affinity for PS. With Annexin V labeled fluorescein as a fluorescent probe and with PI, 7-AAD, DAPI and other nucleic acid dyes, normal cells, early apoptotic cells and late apoptotic cells can be accurately distinguished.
Operation steps :
1. Dilute the binding buffer with deionized water at 1:4 (4ml binding buffer +12ml deionized water);
2. The cells were washed twice with PBS pre-cooled at 4℃, and the cells were re-suspended with 250 UL combined buffer, and the concentration was adjusted to 1× 106/ml;
3. 100μl cell suspension was taken into a 5ml flow tube and 5μl Annexin V/PE and 10μl 7AAD solution were added.
4. Mix well and incubate at room temperature for 15 minutes away from light;
5. 400μl PBS was added to the reaction tube for FACS analysis
(Note: pancreatic enzymes containing EDTA cannot be used when digesting cells)
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
这个能用于带有绿色荧光的细胞吧？凋亡细胞和坏死细胞怎么区分？2022-08-02 12:27:17
Administrator您好，是可以的，例如转染 GFP 等带有绿色荧光信号的细胞样本等检测凋亡都是可以的，Annexin V-PE是橙红色荧光，流式检测建议使用FL2通道，7-AAD是红色荧光建议使用FL3通道检测，荧光通道是不冲突的，做好荧光补偿调节就行；凋亡和坏死的区分坏死细胞膜结构完整性被破坏，所以呈现出双阳性，而凋亡早期膜结构是完整的。