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Mag NHS and Magrose NHS are both magnetic beads with NHS group surface modification, which can form stable peptide bonds with proteins and other molecules with primary amine groups for affinity purification or analysis of antibodies, antigens and other biomolecules. Compared with the traditional carboxyl and amino magnetic beads, the magnetic beads containing NHS groups on the surface do not need to collect EDC/NHS or glutaraldehyde for activation. The biological ligand containing primary amino group can be simply dissolved in the coupling buffer, and the biological ligand can be covalentially coupled to the magnetic bead by mixing the protein solution with Mag NHS magnetic beads for 1~2h at room temperature. It has the advantages of simple operation, mild coupling conditions and fast and efficient biological ligand coupling.
The difference between Mag NHS and Magrose NHS is that Mag NHS is a solid polymer based microsphere with a particle size of 2μm, which is mainly used in the field of analysis and detection (such as in vitro diagnosis, microbial detection, immunoprecipitation, immunocoprecipitation, etc.). Magrose NHS is a natural hydrophilic polymer agarose based porous microsphere, with a particle size of 30~100μm, which has good biocompatibility, large specific surface area and biological ligand coupling amount, mainly used in the field of separation and purification (such as protein separation and purification, antibody separation and purification).
Product features
· Simple, no activation required, can be directly covalently coupled with biological ligand;
· High efficiency, the coupling efficiency of biological ligand can reach more than 90%, which is much higher than that of carboxyl magnetic beads, and the binding load of ligand is higher;
· Rapid, 1~2h can complete the biological ligand coupling;
· Mild, room temperature or 4℃ coupling, coupling system pH 5~9;
· Stable, forming a stable amide bond to prevent ligand from falling off.
· Good biocompatibility, reduce non-specific adsorption.
Figure 1 < p > < img Alt = " SRC ="/images/upload/images / 20180731/20180731110507 _56922. PNG "/ > < / p >Scope of application
It is suitable for the covalent coupling of proteins, antibodies, enzymes, peptides, nucleic acids and other biomolecules containing primary amino groups.
Application direction: In vitro diagnosis, immune detection, cell sorting, immunoprecipitation/co-precipitation, protein/antibody separation and purification.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
