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Mag OH series magnetic microspheres are specially designed for nucleic acid extraction and purification, with a large number of silanol groups (hydroxyl groups) modified on the surface, which can bind the nucleic acid in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples, safe and simple operation. It is very conducive to the automatic and high-throughput extraction of nucleic acid.
Magrose OH is a magnetic agarose microsphere modified with hydroxyl group. Compared with traditional magnetic beads, Magrose OH has faster magnetic response in solution, while maintaining good dispersion, extremely low non-specific adsorption and more abundant binding sites, and can be modified into epoxy, carboxyl, amino and other groups under the action of special chemical reagents. Bioligands such as peptides, proteins and oligonucleotides can be covalently coupled to the surface of microspheres, which is an important carrier tool in medical and molecular biology research.
产品名称 | Mag OH-500 | Mag OH-1000 | Magrose OH |
平均粒径 | 500nm | 1000nm | 30~150μm |
磁核 | Fe3O4 | Fe3O4 | Fe3O4 |
壳层 | 氧化硅 | 氧化硅 | 氧化硅/琼脂糖 |
磁性类型 | 超顺磁性 | 超顺磁性 | 超顺磁性 |
饱和磁化强度 | 40~60 emu/g | 30~50 emu/g | 30~50 emu/g |
应用方向 | 核酸提取和纯化 | ||
操作方式 | 自动/手动 | ||
*水化平均粒径,Malvern Nano测定 |
Product performance
Strong nucleic acid binding ability: > 20μgDNA/mg magnetic beads;
Good operating performance: magnetic beads dispersed uniformly, with superparamagnetism, magnetic response time < 30s;
Good stability and batch repeatability: uniform particle size, polydispersion coefficient < 0.2, showing monodispersion;
Complete silicon oxide coating, stable performance.
Application direction
PCR product purification, plasmid extraction, viral nucleic acid extraction, genomic DNA extraction from blood, tissue, plant and microbial samples
Matters needing attention
1. Magnetic beads are stored in 20% ethanol, freezing, drying and centrifugation operations will cause the agglomeration of magnetic beads, not easy to re-suspension and dispersion, and affect the chemical activity of functional groups on the surface of magnetic beads.
2. Before using this product, be sure to fully oscillate or ultrasonic to keep the magnetic beads in uniform suspension.
3. This product should be used with magnetic separation equipment.
4. This product is for research use only.
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Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
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