Histidine Protein Purification agargo magnetic bead (histidine label protein purification bead) is a new functional material specially designed for efficient and rapid purification of histidine label protein. The high-purity target protein can be purified directly from the biological sample in one step by magnetic separation. The purification process is significantly simplified, the equipment requirements are reduced, and it is suitable for convenient and rapid purification of histidine label protein in scientific research and industrial fields.
Compared with the traditional column Purification method, histidine label Protein was purified by His-tag Protein Purification ball by magnetic separation. There was no need to purify the crude protein sample by several time-consuming centrifugation and filtration steps, no need to load and column chromatography, and no expensive column chromatography equipment. The high yield and purity of the target protein can be easily obtained within 1 hour, and the parallel processing of multiple samples can be easily achieved, significantly improving work efficiency and greatly reducing equipment, time and labor costs.
This product line includes two metal ion chelated magnetic beads, Nickel and Cobalt. They differ in the binding amount of the target protein and the purity of the obtained target protein. It is generally recommended that customers use nickel ion chelated magnetic beads, which can meet the requirements of customers for protein load and purity in most cases. Cobalt ion chelated magnetic beads are recommended for customers with higher purity requirements.
Histidine tag Protein Purification bead is widely used for purification of soluble histidine tag protein secreted or expressed in bacterial, yeast, insect and mammalian cells and denaturen protein.
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