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A novel functional material designed for efficient and rapid purification of Strep-tag II protein. Strep-tag II consists of only eight chemically balanced amino acid short peptides (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), which makes the effect on the structure and activity of the recombinant protein negligible. Therefore, it is not necessary to remove Strep-tag II in the target protein during the purification process. At the same time, the target protein can be purified under physiological conditions. Compared with other tags, these mild purification parameters can preserve the biological activity of the protein and obtain the target protein with a purity of more than 99%.
产品名称 | Magrose Strep-Tactin |
磁珠粒径 | 30-150μm |
配基含量 | -6mg StrepTactin /mL Gel |
蛋白结合量 | -7mg Strep-tag II蛋白/mL Gel① |
悬液浓度 | 10%（v/v) 磁珠悬液② |
保存液 | 1×PBS(0.1%Tween-20+0.05%NaN3) |
保存温度 | 2- 30℃(长期保存，建议置于2- 8℃） |
Binding/Washing Buffer | 10mM Tris-HCl, 150mM NaCl，lmM EDTA, pH8.0 |
Elution Buffer | 2.5mM desthiobiotin in Binding Buffer |
Regeneration Buffer | 0.5MNaOH or 1mM HABA |
Product features
1. The amount of protein binding is related to the characteristics of the target protein, and this is for reference only.
2. 1mL magnetic bead suspension contains 100μl magnetic beads
Application example
Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. 
