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Your Position: Home > Biochemical Reagents > Other Biochemical Reagents > Pi(4,5)P2 Mass Elisa Kit
Pi(4,5)P2 Mass Elisa Kit
Pi(4,5)P2 Mass Elisa Kit
Cat.No：
K-4500
Brand：
Echelon
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
K-4500-1ea Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

The PI(4,5)P2 Mass ELISA detects the amount PI(4,5)P2 extracted from cells by means of a competitive 96-well ELISA assay.

Assay Range:250 to 0.016 pmol PI(4,5)P2

Sample Type:Dried down lipid extracted from cells

Sample Volume:180 µL/sample, run in triplicate

The PI(4,5)P2 Mass ELISA assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4,5)P2 produced.

Once PI(4,5)P2 has been extracted from cell samples, it is incubated with a PI(4,5)P2 detector protein, then added to the PI(4,5)P2-coated microplate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect the PI(4,5)P2 detector protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(4,5)P2 extracted from cells.

Product Background:Phosphatidylinositol 4,5-bisphosphate, or PI(4,5)P2, is a ubiquitous lipid shown to play a central role in a variety of cell functions including the PKB/Akt pathway leading to cell growth, motility, inflammation, and apoptosis. PI(4,5)P2 is a substrate for class I phosphoinositide 3-kinase (PI3-K) and the product of PTEN phosphatase activity.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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