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Your Position: Home > Biochemical Reagents > Other Biochemical Reagents > Pi(3, 4)P2 Mass Elisa
Pi(3, 4)P2 Mass Elisa
Pi(3, 4)P2 Mass Elisa
Cat.No：
K-3800
Brand：
Echelon
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
K-3800-1ea Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

The PI(3,4)P2 Mass ELISA measures the amount of PI(3,4)P2 extracted from cells by means of a competitive ELISA (96 well format).

Assay Range:300 to 1.23 pmol PI(3,4)P2

Sample Type:Dried down lipid extracted from cells

Sample Volume:120 µL/sample, run in duplicate.

The PI(3,4)P2 Mass ELISA is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4)P2 produced. This allows the user to measure the amount of PI(3,4)P2 extracted from cells by means of standard 96 well ELISA format. Eliminating the need for radioactivity and thin layer chromatography.

Once PI(3,4)P2 has been extracted from cellular samples, it is incubated with a PI(3,4)P2 detector, then added to the PI(3,4)P2-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4)P2 detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4)P2 extracted from cells.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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