The PI3-Kinase Activity Fluorescence Polarization Assay determines PI3-K activity through quantification of its product, PI(3,4,5)P3 (PIP3).

Sample Type:in vitro determination of PI3-K activity in cancer tissues and cell lines and compound screening for drug discovery.

Sample Volume:2.5-5.0 µL enzyme / inhibitor

Assay Range:0.0625 µM to 4.0 µM

Product Background:The PI3-Kinase Activity Fluorescence Polarization Assay measures PIP3. Briefly, once PI3-K reaction are complete, the PIP3 detector protein and the fluorescent probe are added. Polarization (mP) values decrease as probe, binding to the PIP3 detector, is displaced by the PIP3 produced by enzymatic activity.

Class I Phosphoinositide 3-kinases (PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PI(4,5)P2 (PIP2) at the 3-hydroxyl of the inositol ring producing PIP3 has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles, PI3-K and PIP3 have become attractive targets for research and drug development in many diseases including inflammation and cancer.

Echelon has multiple formats available for Class III PI3-K. Please see the PI3-K Assay Comparison Guide below for more information.