ColorMixed Protein Marker(15-130KD)

Product Features:

l Three color pre-dyeing, bright color, neat strip, easy to observe.

Low dosage, cost saving, only 5ul can be perfectly presented (1.5mm, 10-hole comb).

l Wide spectrum multi-band, one can meet a variety of experimental needs.

The standard molecular weight protein of this product comes with His tag.

Product Introduction:

This product contains nine color-predyed standard proteins with known molecular weights ranging from 15kD-130kD, each containing approximately 0.2-0.4mg/ml. Prestaining marker can be used to directly observe protein electrophoresis status and clearly judge Western

Transmembrane effect of Blot. After SDS-PAGE gel electrophoresis or transfer to PVDF or NC film, 9 clear color protein strips can be obtained, of which 70kD strips are red, 25kD strips are green, and the rest are blue.

Instruction:

1. This product is a ready-to-use liquid that can be used for direct sample electrophoresis. There is no need to heat, dilute or add reducing agents before loading.

2. After 5ul sample loading, clear rainbow bands can be seen during SDS-PAGE electrophoresis and after film transfer.

3. The recommended separation glue concentration is 15%.

Electrophoretic schematic description:

<img src="/images/upload/image/20180907/20180907185909_43747.png" alt="/>

Note:

1.This product contains a high concentration of glycerin, conventional -20℃ stored as a liquid state, can be used directly. If the temperature of the refrigerator is unstable, resulting in ice, it can be properly subpackaged and stored at 4 ℃ for at least 6 months.

2.The separation effect of Marker is related to the concentration of PAGE glue. If the concentration of separation glue is lower than 15%, the strips below 25kD are not easy to separate, but the green (25KD) and above strips are not affected. If the target strip is less than 25KD, it is recommended that the separation adhesive concentration be greater than or equal to 15%.

3.The transfer effect is related to the transfer time, and it needs to be determined according to the size of the client's target strip. If some of the larger molecular weight strips are not successfully transferred after the transfer, it is normal.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.