CAS:22416-58-6
Purity:HPLC≥97%
Appearance:White to light yellow powder
Storage:Store at 2-8℃,2 years.
TLC
The hydrolysis products were separated on silica gel plate GF254. The solvent system included chloroform, acetic acid, and water (6:7:1. V/V/V). The plate was developed for 2–3 h. After spraying with ethanol: sulfuric acid (95:5, V/V) solvent, the reducing sugars were visualized by heating at 105℃for 5 min.
来源文献:Wen S, Wu G, Wu H. Biochemical characterization of a GH10 xylanase from the anaerobic rumen fungus Anaeromyces robustus and application in bread making. 3 Biotech. 2021 Sep;11(9):406. doi: 10.1007/s13205-021-02956-9. Epub 2021 Aug 11. PMID: 34471589; PMCID: PMC8357908.
HPLC
Xylooligosaccharide analysis was carried out on a HPLC system with a RID-10A detector and an Aminex HPX-42A column (300 mm × 7.8 mm). The column temperature was 60℃. The mobile phase consisted of 0.005 M sulfuric acid at a flow rate of 0.6 mL/min, and the injection volume was 20 μL.
来源文献:[1]Zang, HailianDu, XiaopengWang, JinmingCheng, YiWang, YueSun, ShanshanZhao, XinyueLi, DapengZhang, HuanLi, Chunyan.Insight into cold-active xylanase production and xylan degradation pathways in psychrotrophicAcinetobactersp. HC4 from the cold region of China[J].Cellulose, 2020, 27(13).