CAS:481-42-5
Purity:HPLC≥98%
Appearance:White to yellow Powder
Storage:Store at 2-8℃,2 years.
HPLC
The method was optimized the sample injection volumes were all 20 mL, and the separation was performed on a C18 (4.6×250 mm, 5 mm) column at a flow rate of 1mL/min and a column temperature of 35℃. The mobile phase A was methanol and liquid B was 0.1% H3PO4 (vacuum filtered through a 0.45 mm filter membrane before use). The gradient elution steps were: 40% A, 60% B, 0 min; 75% A, 25% B, 21 min; 90% A, 10% B, 23 min; 90% A, 19% B, 30 min; 40% A, 60% B, 40 min. Plumbagin was detected at 254 nm.
References:
Li Y, Cheng X, Lai J, Zhou Y, Lei T, Yang L, Li J, Yu X, Gao S. ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L. Front Plant Sci. 2023 Jul 3;14:1149669. doi: 10.3389/fpls.2023.1149669. PMID: 37465387; PMCID: PMC10350533.