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Promoter: CMV; Replicons: pUC ori, f1 ori; Terminator: SV40 ploy (A); Vector aliases: pEGFPN1, pEGFPN1; Vector classification: mammalian cell expression vector, fluorescent protein reporter vector; Carrier size: 4733bp; Carrier tag: Enhanced fluorescent protein reporter gene EGFP; Prokaryotic resistance: Kanamycin; Screening marker: Neomycin; Clonal strain: DH5α; Expression host: mammalian cells; Culture conditions: 37℃, LB medium; 5' sequencing primer: CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'; 3' sequencing primer: EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3'; Remarks: There are EcoR I, BamH I and other restriction sites of MCS polyclonal enzyme in the vector. Vector description: pEGFP-N1 encodes a redshift variant of wild-type GFP that has been optimized for brighter fluorescence and higher expression in mammalian cells (maximum excitation wavelength =488nm, maximum release wavelength =507nm). The EGFP gene codes for a sequence that contains more than 190 silent base variations, which correspond to human codon use preferences. One side of the EGFP sequence has been transformed into a translation initiation site consistent with KozaK, further improving the translation efficiency in eukaryotic cells.
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