Save: -20° C Carrier information: Promoter: CMV replication: pUCori,f1ori Terminator: SV40poly(A) Carrier Alias: pEGFPN2, pEGFPN2 carrier Classification: mammalian cell expression vector, fluorescent protein reporter vector Size: 4737bp Carrier tag: C-EGFP prokaryotic resistance: Kanamycin Screening marker: Neomycin cloned strain: DH5α expression host: mammalian cells, culture conditions: 37℃, LB medium, 5' sequencing primer: CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'; 3' sequencing primer: EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3'; Carrier profile: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr
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