Product Introduction：
DiO, is also known as DiOC18(3),which is one of the most commonly used cell membrane fluorescence probes, showing green fluorescence. DiO is a lipophilic membrane dye that can gradually stain the cell membrane of the whole cell by lateral diffusion after entering the cell membrane. DiO fluorescence is very weak before entering the cell membrane. When it is combined with the cell membrane, its fluorescence intensity is greatly enhanced. After being excited, DIO can emit green fluorescence, which has a high quenching constant and excited state lifetime, and can be detected by standard FITC filter. DiO is widely used as a tracer or long-term tracer in forward or reverse direction, living or fixed nerve and other cells or tissues. DiO usually does not significantly affect cell viability.
In addition to cell membrane Fluorescence labeling, DiO can also be used to detect cell fusion and adhesion, cell migration during development or transplantation, and lipid diffusion across the cell membrane by Fluorescence Recovery After Photobleaching (FRAP). Cytotoxicity and labeled lipoproteins are detected. After DiO staining, fixation with paraformaldehyde (no other reagents such as methanol) can be carried out, but the process of permeabilization after staining is not recommended. In addition, plasma membrane staining is also well performed after fixed permeabilization with 0.1% TritonX-100 at room temperature. DiO staining is usually less intense than DiI and is sometimes completely lost in fixed tissues.

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