Calcein-AM/PI，Live/Dead Cell Double Stain Kit
Cat.No：CA1630 Solarbio
Storage：Store at -20℃，avoid light，1 year
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Your Position: Home
> Dye Reagents > Fluorescent Stain > Calcein-AM/PI，Live/Dead Cell Double Stain Kit
Product Information
| Name | Calcein-AM/PI，Live/Dead Cell Double Stain Kit |
| Storage | Store at -20℃，avoid light，1 year |
| Unit | Kit |
| Specification | 500T |
Product Introduction:
Calcein-AM is a cell staining reagent for fluorescent labeling of living cells, emitting green fluorescence (Ex=490nm, Em=515nm). The addition of acetomethoxymethyl ester (AM) group to traditional Calcein increases its hydrophobicity and enables it to easily penetrate the membrane of living cells. Once inside the cell, calcein-AM (which is not fluorescent on its own) is cleaved by intracellular esterases to form the nonpermeable polar molecule Calcein, which is trapped inside the cell and emits strong green fluorescence. Compared with other similar reagents such as BCECF-AM and CFDA, AM is the most suitable fluorescent probe for live cell staining due to its very low cytotoxicity and does not inhibit any cellular functions such as proliferation and lymphocyte chemotaxis.
Because dead cells lack esterase, Calcein-AM is used only for cell viability testing and short-term labeling of living cells. Therefore, Calcein-AM is often used in combination with dead cell fluorescent probes such as propidium iodide (PI) for simultaneous fluorescent double staining of live and dead cells. Propidium iodide (PI) can not pass through the cell membrane of living cells, but can only pass through the disordered region of the dead cell membrane to reach the nucleus and embed into the DNA double helix of the cell to produce red fluorescence (Ex=535 nm, Em=617 nm). Therefore, PI only stains dead cells. Since both Calcein and PI-DNA can be excited by 490 nm, both live and dead cells can be visualized by fluorescence microscopy. However, only dead cells could be observed with 545 nm excitation.
The working principle of this kit is to double dye Calcein-AM and PI to double stain and label live and dead cells, so as to analyze the level of live and dead cells. According to our optimized experimental system, 200μl cell suspension can be stained for 500 times
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Related Products
Sorry, there is no more information.
Related Paper
Implantable,Biodegradable,and Wireless Triboelectric Devices for Cancer Therapy Through Disrupting Microtubule and Actins Dynamics
Click to check >>Author：Shuncheng Yao, Shaobo Wang, Minjia Zheng, Zhuo Wang, Zhirong Liu, Zhong Lin Wang, Linlin Li
IF：29.4000
Publish_to：ADVANCED MATERIALS
PMID：37392034
Self-Activatable Photo-Extracellular Vesicle for Synergistic Trimodal Anticancer Therapy
Click to check >>Author：卢贵红
IF：27.3980
Publish_to：
PMID：33432702
Surface Wettability of Nanoparticle Modulated Sonothrombolysis
Click to check >>Author：吴清渊
IF：27.0000
Publish_to：
PMID：33987928
Liquid Metal Nanoplatform Based Autologous Cancer Vaccines
Click to check >>Author：Dawei Wang, Zhongyang Yu, Yuxia Qi, Kaiwen Hu, Tian Zhou, Jing Liu, Wei Rao
IF：18.0270
Publish_to：ACS Nano
PMID：37253081
Gas therapy potentiates aggregation-induced emission luminogen-based photoimmunotherapy of poorly immunogenic tumors through cGAS-STING pathway activation
Click to check >>Author：Wang Kaiyuan, Li Yang, Wang Xia, Zhang Zhijun, Cao Liping, Fan Xiaoyuan, Wan Bin, Liu Fengxiang, Zhang Xuanbo, He Zhonggui, Zhou Yingtang, Wang Dong, Sun Jin, Chen Xiaoyuan
IF：17.6940
Publish_to：Nature Communications
PMID：37221157
Carbon Nanotubes Promote the Development of Intestinal Organoids through Regulating Extracellular Matrix Viscoelasticity and Intracellular Energy Metabolism
Click to check >>Author：Lin Bao / Chunying Chen
IF：15.8810
Publish_to：ACS Nano
PMID：34622660
Photonic Hyperthermia Synergizes with Immune-Activators to Augment Tumor-Localized Immunotherapy
Click to check >>Author：Tiankuan Li, Zhongqian Hu, Feifei Song, Chenyao Wu, Qizeng Miao, Zhongmin Wang, Wei Feng, Jinhe Guo, Yu Chen
IF：15.3670
Publish_to：Small Methods
PMID：37075769
An orthogonally regulatable DNA nanodevice for spatiotemporally controlled biorecognition and tumor treatment
Click to check >>Author：狄正晗
IF：13.1160
Publish_to：
PMID：32596466
Cyclic reactions-mediated self-supply of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy
Click to check >>Author：张晓娟
IF：12.4790
Publish_to：
PMID：34175561
Injectable multifunctional chitosan/dextran-based hydrogel accelerates wound healing in combined radiation and burn injury
Click to check >>Author：Jintao Shen, Wencheng Jiao, Ziyuan Chen, Chunqing Wang, Xingshuang Song, Lei Ma, Ziyan Tang, Wenrui Yan, Hua Xie, Bochuan Yuan, Chenyun Wang, Jing Dai, Yunbo Sun, Lina Du, Yiguang Jin
IF：10.7230
Publish_to：CARBOHYDRATE POLYMERS
PMID：37321722
Ultra-stretchable,tissue-adhesive,shape-adaptive,self-healing,on-demand removable hydrogel dressings with multiple functions for infected wound healing in regions of high mobility
Click to check >>Author：Kaiyue Liu, Chen Zhang, Rong Chang, Yuanmeng He, Fangxia Guan, Minghao Yao
IF：10.6330
Publish_to：Acta Biomaterialia
PMID：37207743
Engineered extracellular vesicles derived from primary M2 macrophages with anti?inflammatory and neuroprotective properties for the treatment of spinal cord injury
Click to check >>Author：张传杰
IF：10.4350
Publish_to：Journal of Nanobiotechnology
PMID：34789266
Conductive nanocomposite hydrogel and mesenchymal stem cells for the treatment of myocardial infarction and non-invasive monitoring via PET/CT
Click to check >>Author：朱可
IF：10.4000
Publish_to：Journal of Nanobiotechnology
PMID：35524274
Injectable,High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration
Click to check >>Author：Yue Wang, Zuoying Yuan, Yanyun Pang, Daixing Zhang, Guangyu Li, Xu Zhang, Yingjie Yu, Xiaoping Yang, Qing Cai
IF：10.3830
Publish_to：ACS Applied Materials & Interfaces
PMID：37083252
Multifunctional SDF-1-loaded hydroxyapatite/polylactic acid membranes promote cell recruitment, immunomodulation, angiogenesis, and osteogenesis for biomimetic bone regeneration
Click to check >>Author：魏莉
IF：10.0410
Publish_to：
PMID：
NIR-propelled Janus nanomotors for active photoacoustic imaging and synergistic photothermal/chemodynamic therapy
Click to check >>Author：Xiaolei Zhang, Chang Liu, Yangsai Lyu, Ningning Xing, Jia Li, Kun Song, Xiaohui Yan
IF：9.9650
Publish_to：JOURNAL OF COLLOID AND INTERFACE SCIENCE
PMID：37302229
Portable Handheld “SkinPen” Loaded with Biomaterial Ink for In Situ Wound Healing
Click to check >>Author：Fuyuan Zhou, Liangjing Xin, Shuya Wang, Kaiwen Chen, Dize Li, Si Wang, Yuanding Huang, Chuanhang Xu, Mengjiao Zhou, Wenjie Zhong, Huanan Wang, Tao Chen, Jinlin Song
IF：9.5000
Publish_to：ACS Applied Materials & Interfaces
PMID：37262337
Gut microbiota-based PK-PD study and molecular mechanism of Specnuezhenide in the treatment of colorectal cancer targeting carboxylesterase
Click to check >>Author：Ya-Ru Huang, Xi-Xiu Xie, Jing Yang, Xiao-Ying Sun, Xiao-Yun Niu, Cheng-Gang Yang, Ling-Jie Li, Lun Zhang, Dan Wang, Chun-Yu Liu, Sheng-Jie Hou, Chen-Yang Jiang, Yu-Ming Xu, Rui-Tian Liu
IF：8.8000
Publish_to：Cell Reports
PMID：37302068
A Versatile Sunscreen with Minimal ROS Damage and Low Permeability
Click to check >>Author：乔玉春
IF：8.4560
Publish_to：
PMID：31920066
Iron carriers promote biofilm formation and p-nitrophenol degradation
Click to check >>Author：曹利锋
IF：7.0860
Publish_to：Chemosphere
PMID：35033514
Perturbation of gut microbiota plays an important role in micro/nanoplastics-induced gut barrier dysfunction
Click to check >>Author：乔继艳
IF：6.8000
Publish_to：
PMID：33904557
FTH1 Inhibits Ferroptosis Through Ferritinophagy in the 6-OHDA Model of Parkinson’s Disease
Click to check >>Author：田叶
IF：6.0350
Publish_to：
PMID：32959272
Shaoyao Decoction Attenuates DSS-Induced ulcerative colitis, Macrophage and NLRP3 inflammasome activation through the MKP1/NF-κB Pathway
Click to check >>Author：魏媛媛
IF：5.3400
Publish_to：
PMID：34583225
"Tea polyphenol modified
Click to check >>Author： photothermal responsive and ROS generative black
IF：0.0000
Publish_to：10.435
PMID：journal o
Technical Q&A
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.
这个能上流式细胞仪吗？ 2023-05-24 00:17:13
Administrator您好，可以使用流式细胞仪。
可以染细菌吗，效果如何 2023-03-10 20:52:00
Administrator不可以的，如果死活细菌推荐您货号EX3000
用这个试剂染色后多长时间内拍摄荧光最好呢？如果放的时间长了会有什么影响呢 2022-09-30 00:43:45
Administrator您好，染色后都是立即进行荧光检测的，荧光标记会有淬灭性，淬灭速度与环境光源强度有关，如果没办法立即检测尽量做避光保存或者制片时加抗荧光衰减封片剂尽可能减缓淬灭速度。