| CAS |
312756-74-4 |
| Chinese Name |
4-溴-N-(4-溴苯基)-3-[[(苯基甲基)氨基]磺酰基]苯甲酰胺 |
| English Name |
RS-1 |
| Synonyms |
RAD51-stimulatorycompound1 |
| Molecular Formula |
C20H16Br2N2O3S |
| Molecular Weight |
524.23 |
| Solubility |
Soluble in DMSO |
| Purity |
≥98% |
| Appearance |
White to off-white Solid |
| Storage |
Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| Delivery Time |
1-2 Days |
| MDL |
MFCD00348720 |
| SMILES |
O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2 |
| InChIKey |
SWKAVEUTKGKHSR-UHFFFAOYSA-N |
| InChI |
InChI=1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25) |
| PubChem CID |
1039737 |
| Target Point |
RAD51 |
| Passage |
Cell Cycle;DNA Damage/DNA Repair |
| Background |
RS-1 is an activator of RAD51 that also enhances CRISPR/Cas9 activity. |
| In Vitro |
RS-1 is a RAD51 activator，stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor，and such an effect is not via inhibiting its ATPase activity. RS-1(20 μM)affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1(0，1，5，10，15，20，and 25 μM)stimulates strand assimilation activity of hRAD51. RS-1(7.5 μM)promotes resistance of human cells to cross-linking chemotherapy[1]. RS-1(0，7.5，15 μM)increases Cas9-mediated knock-in efficiencies in rabbit embryos[2]. |
| Kinase Experiment |
Briefly，15 μL reaction volumes include a DNA strand exchange protein(0.8 μM)that is preincubated for 5 min at 37°C with 1 μM(nucleotide concentration)32P-labeled oligonucleotide 306.7 in a reaction buffer containing 20 mM Hepes(pH 7.5)，1 mM DTT，2 mM nucleotide cofactor，and 1 mM MgCl2 and various concentrations of RS-1. For experimental buffer conditions that included calcium，1 mM CaCl2 is present in addition to(in the case of hRAD51)or in the place of(in the case of RecA and scRAD51)the 1 mM MgCl2. Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding reaction，10 μL of 19.75 μM(base pair concentration)supercoiled homologuecontaining target plasmid DNA(pRS306)is next added along with sufficient magnesium acetate to give a final concentration of 10 mM[1]. |
| Data Literature Source |
[1]. Jayathilaka K，et al. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53.
[2]. Song J，et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. |
| Unit |
Bottle |
| Specification |
5mg 10mM*1mL in DMSO 10mg |
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