It is used in immunoassay, complement activation and other experiments. The hemolysis test involves mixing antibodies against erythrocyte (commonly called hemolysin) with the corresponding erythrocyte, which are dissolved when complements are present. This specific hemolysis is visible and is widely used in the determination of serum total complement activity and the functional activity of individual complement components. In addition, hemolysis test is often used as an index system in complement fixation test. The principle of complement fixation test (CFT) is that the complement has no specificity and can bind to any kind of antigen-antibody complex, but when the antigen and antibody do not correspond, the complement does not bind and exists free. At this time, as in the above reaction system. The addition of sheep erythrocyte (antigen) and hemolysin (antibody) system can combine with free complements to produce hemolysis. Therefore, sheep erythrocyte and hemolysin are the indicator system of CFT (also known as hemolysis system). According to the results of the test, we can know whether there is a corresponding antigen or antibody in the detection system according to whether the hemolysis occurs.

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