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CAS:10030-85-0
Purity:HPLC≥98%
Appearance:White to off-white powder
Storage:Store at 2-8℃，2 years.
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HPGPC
A Shimadzu LC-20AT chromatography unit equipped with a Shodex OH pak SB-804 column (8 × 300 mm i.d., YMC Co., Ltd, Kyoto, Japan) and a refractive index detector (Shimadzu RID-10 A) was employed in these studies.Briefly, pectin (5 mg) was dissolved in distilled water (1 mL) and injected into the column. Distilled water was used as the mobile phase, and the flow rate was 0.8 mL/min. The column temperature was maintained at 30 ℃. The injection volume was 20 μL.
References：
[1] A Y C , A Y W , A L X ,et al.Ultrasound-assisted modified pectin from unripe fruit pomace of raspberry ( Rubus chingii Hu): Structural characterization and antioxidant activities[J].LWT, 134[2023-10-11].
HPLC
Chromatographic conditions as followed: Zorbax Eclipse Plus-C18 (4.6 mm × 100 mm, 5 μm, Agilent, USA) capillary column, ultraviolet detector (250 nm detection wavelength), mobile phase consisting of 0.025 M phosphate buffer (pH 6.7) with 83% (solution A) and 17% acetonitrile (solution B) in water, 0.5 mL/min flow rate and column at 30 ℃.
References：
Yang B, Wu Q, Luo Y, Yang Q, Wei X, Kan J. High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic. Int J Biol Macromol. 2019 Sep 15;137:676-687. doi: 10.1016/j.ijbiomac.2019.07.034. Epub 2019 Jul 5. PMID: 31284006.
HPLC
4 μL of the filtrate was analyzed using an Agilent 1260 Series HPLC system (Agilent Technologies, CA, USA). Chromatographic conditions were as follows: Zorbax Eclipse Plus-C18 (4.6 mm × 100 mm, 5 μm, Agilent, USA) capillary column, ultraviolet detector (250 nm detection wavelength), mobile phase consisting of 0.025 M phosphate buffer (pH 6.7) with 83% (solution A) and 17% acetonitrile (solution B) in water, 0.5 mL/min flow rate and column temperature at 30 ℃.
References：
Yang B, Wu Q, Luo Y, Yang Q, Chen G, Wei X, Kan J. Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities. Int J Biol Macromol. 2019 Aug 1;134:631-644. doi: 10.1016/j.ijbiomac.2019.05.079. Epub 2019 May 15. PMID: 31100397.
