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TBE buffer is a common buffer salt solution for nucleic acid electrophoresis in biology, which is mainly used for DNA agarose gel electrophoresis. The main components of TBE are TRIS-Borate and EDTA, which has strong buffer capacity, is suitable for longer electrophoresis time, high resolution, and good separation effect when electrophoresis fragments less than 1kb. The boric acid content of TBE buffer can affect the efficiency of DNA recovery and the subsequent enzyme reaction, so TAE buffer is recommended for DNA fragment recovery experiments by agar-gel electrophoresis.
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10×TBE缓冲液无RNase污染吗2022-09-30 08:58:19
Administrator没有做过去除RNA酶的特殊处理