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CAS:520-36-5
Appearance:Light yellow to yellow powder
Storage:-20℃
Purity:HPLC≥98%
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HPLC
Apigenin, scutellarein, scutellarin, and apigenin-7-O-glucuronide were measured at 335 nm using a Kinetex 5 μm Biphenyl 100 ? LC Column (250 × 4.6 mm; Phenomenex, USA) operating at 30 ℃. The mobile phase consisted of 0.1% formic acid and acetonitrile with methanol at a flow rate of 1 ml min?1 using the following gradients: 0–20 min, 22% acetonitrile, 5% methanol; 20–22 min, acetonitrile increased from 22 to 90%; 25 min, 22% acetonitrile. Subsequently, the column was washed and equilibrated for 5 min before the next injection. Thirty microliters of the sample were injected into the HPLC system and each run was stopped at 30 min after the injection.
References：
Liu X, Cheng J, Zhang G, Ding W, Duan L, Yang J, Kui L, Cheng X, Ruan J, Fan W, Chen J, Long G, Zhao Y, Cai J, Wang W, Ma Y, Dong Y, Yang S, Jiang H. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches. Nat Commun. 2018 Jan 31;9(1):448. doi: 10.1038/s41467-018-02883-z. PMID: 29386648; PMCID: PMC5792594.
UPLC-MS
Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was performed with liquid mass spectrometer for evaluation. The determination was performed on the SB-C18 column at the flow rate of 0.3 mL/min and the column temperature was 40 ℃. The flavonoid and anthocyanin components were detected at 210 nm.
References：
Shi M, Ali MM, He Y, Ma S, Rizwan HM, Yang Q, Li B, Lin Z, Chen F. Flavonoids Accumulation in Fruit Peel and Expression Profiling of Related Genes in Purple (Passiflora edulis f. edulis) and Yellow (Passiflora edulis f. flavicarpa) Passion Fruits. Plants (Basel). 2021 Oct 20;10(11):2240. doi: 10.3390/plants10112240. PMID: 34834602; PMCID: PMC8620868.
