CAS:73-03-0
Storage:Powder:-20℃,2 years;Insolvent(Mother Liquid):-20℃,6 months;-80℃,1 year
Purity:HPLC≥98%
Appearance:White to off-white Solid
Examples of using this product(for reference only)
In Vitro:
Arabidopsis(14 day old,Col-0, dcp5-1 and ssf-2 seedlings;1 mM Cordycepin):
Briefly, 14-day-old Col-0, dcp5-1 and ssf-2 seedlings were incubated in 3 mL of incubation buffer (1 mM Tris–HCl pH 7.5, 1 mM NaCl, 1 mM KCl, 15 mM sucrose) with rotation at 75 rpm in 12-well plates for 15 min. The incubation solution was then replaced with 3 mL of fresh buffer containing 1 mM cordycepin (Solarbio, Beijing, China, cat: 70-03-0). Samples incubated at 22 ℃ were harvested at different time points (0.5, 1, 2, 4, 6, and 12 h), frozen in liquid nitrogen, and subjected to total RNA isolation and RT-qPCR analysis as de_x005f scribed above.
Reference:
Wang W, Wang C, Wang Y, Ma J, Wang T, Tao Z, Liu P, Li S, Hu Y, Gu A, Wang H, Qiu C, Li P. The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis. Plant Cell. 2023 May 23:koad151. doi: 10.1093/plcell/koad151. Epub ahead of print. PMID: 37220754.