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CAS:25389-94-0
Appearance:White Powder
Storage: 2-8℃
Purity:potency(Anhybrous)(mcg/mg)≥750
Product Introduction:
Kanamycin sulfate is an aminoglycoside antibiotic that inhibits both gram-positive and Gram-negative bacteria and mycoplasma. The mechanism of action is to target binding to the bacterial 70S ribosome subunit, thereby inhibiting ribosome translocation and causing miscoding to further interfere with protein synthesis.
Description:
Kanamycin sulfate is an aminoglycoside antibiotic that binds to the 70S ribosomal subunit to inhibit peptide chain formation and is used for resistance screening.
Use and synthesis method:
Kanamycin sulfate can be used to inhibit bacterial contamination in cell culture. In molecular biology studies, it is commonly used to select bacterial clones that successfully transform kanamycin resistance gene (kan gene). It is also commonly used in Agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying the npt II (APH3) gene. The product is soluble in water (50 mg/ml) and should be used after filtration and sterilization. The recommended working concentration is 30-100μg/ml.
Physical shape:
Moisture (%) : ≤4.0
pH (1%, water) 25℃ : 6.5-8.5
Ash (%) : ≤1.0
Solubility:
Soluble in water, 10-50mg/ml as mother liquor, stored at 2-8 ℃
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
该抗生素用于选择培养基（添加琼脂）时，对温度有何要求（70摄氏度能否添加）2023-07-20 21:56:32
Administrator您好，在培养基中添加硫酸卡那霉素时，需要培养基不烫手时添加，以防温度过高抗生素失效