{{cart_num}}
My CartReactivity：Human
Application：IP,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1639
Host：Mouse
Qty：
Size：
| Name | Anti-DCTN1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 3D5-C6-D5 |
| Dilution Ratio | WB 1:500-1000. IP 1:10-20. |
| Target | DCTN1 |
| Background | This gene encodes the largest subunit of dynactin,a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150kD.Dynactin binds to both microtubules and cytoplasmic dynein.Dynactin is involved in a diverse array of cellular functions,including ER-to-Golgi transport,the centripetal movement of lysosomes and endosomes,spindle formation,chromosome movement,nuclear positioning, and axonogenesis.This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus.Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).[provided by RefSeq, Oct 2008] |
| Swiss Prot | Q14203 |
| Gene ID | 1639 |
| Subcellular Location | Cytoplasm cytoskeleton |
| Immunogen | Recombinant protein of DCTN1 |
| Calculated MW | 127/137/139/141/142kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DCTN1 antibody diluted at 1:1000;Lane: 293T,HeLa,MCF7,K562.
Immunoprecipitation analysis of HeLa extracts using DCTN1 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
