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My Cart> Antibodies > Loading Control Antibodies > Mitochondria > Anti-COX4I1 Monoclonal AntibodyReactivity：Mouse,Rat,Human,Hamster,Monkey,Goat
Application：IP,ICC,FCM,IF,IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1327
Host：Mouse
Qty：
Size：
| Name | Anti-COX4I1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 4D11-B3-E8 |
| Dilution Ratio | WB 1:1000. IHC 1:50-200. IF 1:100-200. IP 1:20. FCM 1:100. ICC 1:100-200. |
| Target | COX4I1 |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane.The complex consists of 13 mitochondrial- and nuclear-encoded subunits.The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities.The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation,and shares a promoter with it.[provided by RefSeq,Jul 2008] |
| Swiss Prot | P13073 |
| Gene ID | 1327 |
| Subcellular Location | Cytoplasm Mitochondrion inner membrane |
| Immunogen | A synthetic peptide of human COX4I1 |
| Calculated MW | 20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:100-200. IP 1:10-20. FCM 1:50-100. |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human Hamster Monkey Goat |
| Application | IP ICC FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with COX4I1 antibody diluted at 1:1000;Lane: Mouse skeletal muscel Jurkat.
Immunohistochemistry of paraffin-embedded Human colorectal carcinoma with COX4I1 antibody diluted at 1:100.
Immunofluorescence analysis of HeLa using COX4I1 antibody diluted at 1:100.
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