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My CartReactivity：Human
Application：ICC,ChIP,WB,IF
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1938
Host：Mouse
Qty：
Size：
| Name | Anti-eEF2 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 4B3-G7-H5 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ChIP 1:20. ICC 1:50-200. |
| Target | eEF2 |
| Background | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| Swiss Prot | P13639 |
| Gene ID | 1938 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human eEF2 |
| Calculated MW | 95kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | ChIP ICC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with eEF2 antibody diluted at 1:1000;Lane: HL-60,Jurkat,SH-SY5Y,U20S,HeLa.
Immunofluorescence analysis of HeLa cells using eEF2 antibody diluted at 1:100.
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