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My CartReactivity：Human
Application：WB,IHC,IP,IF,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5591
Host：Mouse
Qty：
Size：
| Name | Anti-PRKDC Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 6D1-C11-F10 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | PRKDC |
| Background | This gene encodes the catalytic subunit of the DNA-dependent protein kinase(DNA-PK).It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination.The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq,Jul 2010] |
| Swiss Prot | P78527 |
| Gene ID | 5591 |
| Subcellular Location | Nucleus nucleolus |
| Immunogen | Recombinant protein of human PRKDC |
| Calculated MW | 469kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | ICC IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PRKDC antibody diluted at 1:1000;Lane: K562,HeLa,HCT-116,A549,MOLT-4.
Immunohistochemistry of paraffin-embedded Human breast cancer with PRKDC antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using PRKDC antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using PRKDC antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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