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My CartReactivity：Mouse,Rat,Monkey,Human
Application：WB,IF,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5315
Host：Mouse
Qty：
Size：
| Name | Anti-PKM Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 1A7-G6-H6 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
| Target | PKM |
| Background | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011] |
| Swiss Prot | P14618 |
| Gene ID | 5315 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human PKM |
| Calculated MW | 60kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.4. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Monkey Human |
| Application | ICC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PKM antibody diluted at 1:1000;Lane: COS7,PC12,C6,NIH3T3,HeLa.
Immunofluorescence analysis of HeLa cells using PKM antibody diluted at 1:100.
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