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My CartReactivity：Mouse,Human
Application：IP,IF,WB,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1737
Host：Mouse
Qty：
Size：
| Name | Anti-DLAT Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 4A4-B6-C10 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. |
| Target | DLAT |
| Background | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Swiss Prot | P10515 |
| Gene ID | 1737 |
| Subcellular Location | Mitochondrion matrix |
| Immunogen | Recombinant protein of human DLAT |
| Public Immunogen Range | 242-426aa |
| Calculated MW | 69kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | IP IF WB ICC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DLAT antibody diluted at 1:1000;Lane: Jurkat,A549,U251,F9,LNCaP,HeLa.
Immunofluorescence analysis of HeLa cells using DLAT antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using DLAT antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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Redox Homeostasis Disruptors Based on Metal-Phenolic Network Nanoparticles for Chemo/Chemodynamic Synergistic Tumor Therapy through Activating Apoptosis and Cuproptosis
Click to check >>Author：Fan Zhao, Hongyan Yu, Liying Liang, Chen Wang, Dier Shi, Xiangyu Zhang, Yao Ying, Wei Cai, Wangchang Li, Juan Li, Jingwu Zheng, Liang Qiao, Shenglei Che, Jing Yu
IF：10.0000
Publish_to：Advanced Healthcare Materials
PMID：37369362
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