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My CartReactivity：Human
Application：IP,ICC,WB,IF
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5747
Host：Mouse
Qty：
Size：
| Name | Anti-PTK2 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 3D8-H11-E12 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | PTK2 |
| Background | This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. |
| Swiss Prot | Q05397 |
| Gene ID | 5747 |
| Subcellular Location | Cell junction Cell membrane Cytoplasm Cytoplasmic side Nucleus Peripheral membrane protein cell cortex centrosome cytoskeleton focal adhesion microtubule organizing center |
| Immunogen | Recombinant protein of human PTK2 |
| Calculated MW | 40/49/63/99/121/114/119kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IP ICC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PTK2 antibody diluted at 1:1000;Lane: COS7,HUVEC,A549,HeLa.
Immunofluorescence analysis of HeLa cells using PTK2 antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using PTK2 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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