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My Cart> Antibodies > Loading Control Antibodies > Nucleus > Anti-PCNA Monoclonal AntibodyReactivity：Mouse,Rat,Human,Hamster,Monkey
Application：ICC,IHC,IF,IP,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5111
Host：Mouse
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| Name | Anti-PCNA Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 2E1-G10-H10 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:100-200. IP 1:10-20. ICC 1:50-200. |
| Target | PCNA |
| Background | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq,Jul 2008] |
| Swiss Prot | P12004 |
| Gene ID | 5111 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human PCNA |
| Calculated MW | 29kDa |
| Purification Method | Fractional precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human Hamster Monkey |
| Application | ICC IHC IF IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PCNA antibody diluted at 1:1000;Lane: CHO-K1 C6 COS7 3T3 HeLa.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with PCNA antibody diluted at 1:400
Immunofluorescence analysis of HeLa using PCNA antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using PCNA antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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