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My CartReactivity：Mouse,Rat,Human
Application：IF,ICC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：79084
Host：Mouse
Qty：
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| Name | Anti-WDR77 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 8A10-C10-E8 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
| Target | WDR77 |
| Background | WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061).This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al.,2002 [PubMed 11756452]).[supplied by OMIM,Mar 2008] |
| Swiss Prot | Q9BQA1 |
| Gene ID | 79084 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human WDR77 |
| Calculated MW | 37kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | IF ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with WDR77 antibody diluted at 1:1000;Lane: C6,K562,NIH3T3.
Immunofluorescence analysis of HeLa cells using WDR77 antibody diluted at 1:100.
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